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《Inspection and Quarantine Science》 2005-01
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THE COMPARISON FOR DETECTION OF PSP IN THE PRODUCT OF SHELLFISH BY ELISA AND BY BIOLOGICAL METHOD USING MICE

Yu Bing~*,Cao Jijuan,You Yongli~*,Ma Lidan~*,Yu Ke,Zhao Xin~* (Dandong Entry-Exit Inspection and Quarantine Bureau) (Liaoning Entry-Exit Inspection and Quarantime Bureau) Wang Dianfu(Liao Dong College)  
This experiment has compared the results from ELISA and from biological method using mice for detection of PSP content in the product of shellfish. The results indicated two methods were identical extremely. But when the samples contained a high amount of PSP, the results were different to a certain extent. By reason that ELISA was more suitable for detecting low PSP content. In addition, this article has used ELISA for detection of PSP content in the product of shellfish. Ten kinds of Fifty two samples were detected quantitatively. The results indicated that the samples ratio were approximately 15%. which PSP content were more than safe limit, that was 80μg/100g,. The sensitivity of the ELISA method was lower than that of others. The assay was very specific, and furthermore, the positive results were identical with those using other methods. The established method was exactly fit for detecting shellfish poison rapidly.
【Fund】: 国家“十五”重大科技专项“食品安全关键技术研究”中“贝类毒素检测新技术的研究”(课题编号 :2 0 0 1BA80 4A2 0 -2 )中的部分内容
【CateGory Index】: Q503
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