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《Inspection and Quarantine Science》 2005-01
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FLUOROGENIC ASSAY FOR RAPID DETECTION OF Vibrioparahaemolyticus IN AQUATIC PRODUSTS

Gao Qili,Zhang Xia,Cai Guorui,Zhang Haiying (Tianjin Entry-Exit Inspection and Quarantine Bureau)  
A novel fluorogenic assay for the convenient and rapid detection of Vibio parahaemolyticus in Aquatic Products was developed.In the assay,the enrichment of Vibio parahaemolyticus was carried out in arabinose-glucuronate medium(0.5% arabinose,0.25%glucruonate,0.1%polypeptone,0.1%ammonium sulfate,2%Nacl,2ug of polymyxin Bsulfate per mL,pH 8.5)at 37℃,6h.After the cultivation,the trypsinlike activity of the bacteria was measured by fluorescence with the fluorogenic substrate benzoyl-L-arginine-7-aminomethyl-coumarin.Even in the presence of 4×10~5 cells of Vibrio alginolyticus,20 cells of Vibio parahaemolyticus were clearly detected by the assay.Eighteen contaminated samples of 5 Aquatic products were detected for Vibio parahaemolyticus by the fluorogenic assay after enrichment.The results were compared with those obtained by the conventional most-probable-number method.There was a linear relationship between trypsinlike activity measured by the assay and the number of Vibrio parahaemolyticus cells in Aquatic products as determined by the most-probable-number method.The coefficient was 0.86.
【CateGory Index】: TS254
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