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《Journal of Xiamen University(Natural Science)》 1987-02
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Studies on the Redox Properties of Nitrogenase from Azotobacter vinelandii

Yu Changfeng Zeng Ding ( Department of Biology )  
The redox properties of nitrogenase complex and its components from Azotobacter vinelandii have been studied by using optical titration. stepwise potentiostatic electrolysis and controlled potential coulometry. It was found that Av2 has a midpoint potential (Em)=-265±22 mV, with one electron transferred. When MgATP is present, the Em is lowered to -378±20 mV and two electrons are transferred. MgADP can also lower the Em to -326 mV only one electron is transferred in the redox process. Avl shows two redox regions with Em=-36 mV and -326mV. With the presence of ATP generating system, the Avl-Av2 complex has two redox regions, Em2=-290 mV and Em2=-452 mV, corresponding to transferring 6.5 and 5.1 electrons respectively.
【Key Words】： Redox properties Nitrogenase Azotobacter vinelandii
【Fund】： 中国科学院科学基金
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【References】
Chinese Journal Full-text Database 1 Hits
1 Huang Heqing Xu Liangshu Chen JiapanZhang Fengzhang Zeng Ding(Dept. of Biol.);Effect of Potential Treatment on Nitrogenase Activity of Azotobacter vinelandii Whole Cell[J];Journal of Xiamen University(Natural Science);1989-05
【Citations】
Chinese Journal Full-text Database 2 Hits
1 Xu Liang-shu, Zeng Ding, Lin Qing-mu, Chen Jia-ban, Zhang Feng-zhang (Dept. of Biology);Studies on the Separation and Crystallization of Mo-Fe Protein front Azotobacter Vinelandii and the Estimation of Its Redox potential[J];Journal of Xiamen University(Natural Science);1979-04
2 Chen Hongbo Zhang Hongtu Lin Guodong Tsai K.R. (Department of Chemistry, Institute of Physical Chemistry);Chemical Modeling of ATP-driven Electron Transport in Nitrogenase Catalyzed Reactions Ⅰ.The Studies of Complexing [Fe_4S_4(SR)_4]~(2-) Cluster with ATP Using Polarographic and Electronic Absorption Spectroscopic Methods[J];Journal of Xiamen University(Natural Science);1985-04
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 HuangHe-Qing;Zhang Feng-Zhang;Xu Liang-Shu;Zeng Ding(Departmem of Biology,Xiamen Universdy,Xiamen 361005)Gerald D.Watt(Department of Chemistry,Bwr,UT 84602,USA);Studies on Physical-Chemical Characterization of Fe-S Clusters from Nitrogenase by Spectral Technique[J];;1996-04
2 Chen Jafan zhang FengZhang Huang Heqing Xu Liangshu ( Nitrogen Fixation Research Unit, Xiamen Univ.);Acetylene Reduction Activities of Immobilized Azotobactor vcnilandii-OP Cell[J];Journal of Xiamen University(Natural Science);1987-02
3 Huang Heqing Xu Liangshu Chen JiapanZhang Fengzhang Zeng Ding(Dept. of Biol.);Effect of Potential Treatment on Nitrogenase Activity of Azotobacter vinelandii Whole Cell[J];Journal of Xiamen University(Natural Science);1989-05
4 Zhang Fengzhang Zhang Hongtu Liu AiminXu Liangshu Yang Ru Wan Hulin (Nitrogen Fixation Research Group, Dept. of Chem, & Dept. of Biol. );Effects of O-Phthalaldehyde on Nitrogenase Catalysis and Self-Assembling of Mo-Fe-S Cluster Complexes[J];Journal of Xiamen University(Natural Science);1993-06
5 Zhang Fengzhang; Yin Bei; Huang Heqing;Long Minnan; Xu Liangshu; Zeng Ding(Dept. of Biol. );Effect of Homocitrate on Reconstitution Activity of FeMoco from Nitrogenase[J];JOURNAL OF XIAMEN UNIVERSITY(NATURAL SCIENCE);1996-01
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 Xu Liang-shu, Zeng Ding, Lin Qing-mu, Chen Jia-ban, Zhang Feng-zhang (Dept. of Biology);Studies on the Separation and Crystallization of Mo-Fe Protein front Azotobacter Vinelandii and the Estimation of Its Redox potential[J];Journal of Xiamen University(Natural Science);1979-04
2 Zhuang Xiu-zhi Liu Wen-yuan Yang Ru Lin Pei-san (Nitrogen Fixation Research Unit, Xiamen University; Institute of Phsical Chemistry, Xiamen University);Chemical Modeling of Biological Nitrogen FixationⅦ The Determination of Molybdenum, Iron and Sulfur in FeMo-co Modeling Compounds[J];Journal of Xiamen University(Natural Science);1980-02
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 HuangHe-Qing;Zhang Feng-Zhang;Xu Liang-Shu;Zeng Ding(Departmem of Biology,Xiamen Universdy,Xiamen 361005)Gerald D.Watt(Department of Chemistry,Bwr,UT 84602,USA);Studies on Physical-Chemical Characterization of Fe-S Clusters from Nitrogenase by Spectral Technique[J];;1996-04
2 HUANG He-qing 1,2 ,ZHAI Wen-jing 1,LIN Qing-mei 3,4 ,XIE Wei-hu 1,SUN Yao-dong 1 (1 Division of Biotechnology, Department of Biology; 2.The Key lab. of MOE for Cell biology and Tumor Cell Engineering; 3 Research Center of Environment Scienc;Primary studies on electro-inducing Azotobacter vinelandii to express characteristics of ammonium-resistant[J];BIOTECHNOLOGY;2000-02
3 HUANG He-Qing 1,3 SUN Yao-Dong 1 LIN Qing-Mei 2 TA NG Hua 1 ZHAI Wen-Jing 1 (Department of Biology 1,Research Center of Environment Sci ence 2,State Key Lab.for Phys.Chem. of Solid Surface and Department of Chemistry 3,Xiamen University,;EFFECT OF ELECTRON DONOR AND ACCEPTOR ON CAPACITIES OF AMMONIA-RESITANT FROM AZOTBACTER VINELANDII[J];Microbiology;2001-04
China Proceedings of conference Full-text Database 2 Hits
1 Huang He-Qing Zhai Wen-Jing Lin Qing-Mei Zhang Feng-Zhang Xie Wei-Hu Sun Yao-Dong(Department of Biology, Research Center of Environment Science , Xiamen University, Xiamen, 361005);Studies on electro-inducing strain of Azotobacter vinelandii with Ammonium-resistant by cyclic voltammetry[A];[C];1999
2 Huang He-Qing Sun Yao-Dong Zhang Feng-Zhang Lin Qing-mei(Deprtment of Biology , Research Center of Environment Science,Xiamen University,Xiamen 361005);Effect of electronic donor and receptor on capacity for expressing activity of nitrogen fixation from Azotbacter vinelandii[A];[C];1999
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