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《Journal of Xiamen University(Natural Science)》 1987-02
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Acetylene Reduction Activities of Immobilized Azotobactor vcnilandii-OP Cell

Chen Jafan zhang FengZhang Huang Heqing Xu Liangshu ( Nitrogen Fixation Research Unit, Xiamen Univ.)  
C2H4 reduction activities of Azotobacter venilandii-OP cells immobilized by several methods including entrapment within alginate gel, agar gel, gelatin and adsorption to ion exchange cellulose, have been compared. Sodium alginate entrapment has been proved to be the best. Specific activity of the alginate-immobilized cells was estimated to be 11.8 μml/h/1010 cells in our hands. C2H2 reduction activity could be demonstrated up to fifteen days. Optimal conditions for C2H2 reduction appeared to be 30℃, pH7.5, and a Km value of 20μM was obtained. Effect of some respiration inhibitors, nitrogenous salts and CO2 on C2H2 reduction activity have also been examined.
【Key Words】： Acetylene reduction Azotobacler venilandii Immobilization
【Fund】： 中国科学院科学基金
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【References】
Chinese Journal Full-text Database 1 Hits
1 Liu Hong Xu Liangshu Zeng Ding Zhang Fengzhang(Department of Biology);Preliminary Studies on the Relationship between H_2- uptaking Gene and Plasmid DNA from some Strains of Rhizobinm arachis[J];Journal of Xiamen University(Natural Science);1991-02
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Xu Liang-shu, Zeng Ding, Lin Qing-mu, Chen Jia-ban, Zhang Feng-zhang (Dept. of Biology);Studies on the Separation and Crystallization of Mo-Fe Protein front Azotobacter Vinelandii and the Estimation of Its Redox potential[J];Journal of Xiamen University(Natural Science);1979-04
【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 Jia Demin and Wei Xiuping (Materials Science Research Institute, South China University of Technology, Guangzhou);STUDIES ON A NEW PHOTO-CROSSLINKED POLYURETHANE AS THE CARRIER OF IMMOBILIZED CELLS[J];Polymeric Materials Science & Engineering;1990-03
2 Shang Dejing(The Food Biochemical Institute of Liaoning Normal University Dalian 116029);THE STUDY OF IMMOBILIZED HERICIUM ERIENCE CELL[J];JOURNAL OF LIAONING NORMAL UNIVERSITY(NATURAL SCIENCE EDITION);1995-04
3 Shang Dejing (Department of Biology, Liaoning Normal University, Dalian, 116029);Study on Immobilization of Hericium erience Cell[J];Food and Fermentation Industries;2002-01
4 Yu Changfeng Zeng Ding ( Department of Biology );Studies on the Redox Properties of Nitrogenase from Azotobacter vinelandii[J];Journal of Xiamen University(Natural Science);1987-02
5 Huang Heqing Xu Liangshu Chen JiapanZhang Fengzhang Zeng Ding(Dept. of Biol.);Effect of Potential Treatment on Nitrogenase Activity of Azotobacter vinelandii Whole Cell[J];Journal of Xiamen University(Natural Science);1989-05
6 Zhang Fengzhang Zhang Hongtu Liu AiminXu Liangshu Yang Ru Wan Hulin (Nitrogen Fixation Research Group, Dept. of Chem, & Dept. of Biol. );Effects of O-Phthalaldehyde on Nitrogenase Catalysis and Self-Assembling of Mo-Fe-S Cluster Complexes[J];Journal of Xiamen University(Natural Science);1993-06
7 Zhang Fengzhang; Yin Bei; Huang Heqing;Long Minnan; Xu Liangshu; Zeng Ding(Dept. of Biol. );Effect of Homocitrate on Reconstitution Activity of FeMoco from Nitrogenase[J];JOURNAL OF XIAMEN UNIVERSITY(NATURAL SCIENCE);1996-01
8 WANG Qing;LIU Tao;CHEN Shan;Department of Food Science ,Xinyang College of Agriculture and Forestry;Center for Sugar Engineering and Technology Research,Guangxi University;;The influence of carbon and nitrogen source on the molecular weight of dextran produced by immobilized Leuconostoc mesenteroides[J];China Food Additives;2015-04
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