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《JOURNAL OF XIAMEN UNIVERSITY(NATURAL SCIENCE)》 2000-01
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Kinetics of Inactivation of β-glucosidase from Ampullarium crossean in Urea Solutions

CHEN Qing Xi, SHI Yan, ZHANG Zhe, HUANG Jian She, ZHUANG Zong Lai (Dept. of Biol., Xiamen Univ., Xiamen 361005 ,China)  
The inactivation of β glucosidase from Ampullarium crossean has been studied using the kinetic method of the substrate reaction during denaturation in urea solutions.The microscopic rate constants of inactivation for the free enzyme and the enzyme substrate complex were determined. Comparison of these rate constants indicates that the presence of substrate offers marked protection of this enzyme against inactivation by urea.
【Fund】: 福建省自然科学基金!(C97005)
【CateGory Index】: Q502
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