Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Xiamen University(Natural Science)》 2010-01
Add to Favorite Get Latest Update

Preparation of Bovine Serum Albumin Affinity Monolith for Chiral Separation of D,L-tryptophan by Capillary Electrochromatography

YAO Chuan-yi,JIA Jing-bo,CHEN Wei-qi (College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China)  
Enantioseparation of D,L-tryptophan was studied on an affinity monolithic column by capillary electrochromatography. A chitosan/silica hybrid monolithic column was prepared by the sol-gel method.Then the affinity stationary phase was synthesized by the in situ covalent immobilization of BSA on the monolithic column activated with glutaraldehyde.The monolith was used for chiral separation of D,L-tryptophan by capillary electrochromatography.The effects of pH,concentration of running buffer,and the content of organic modifier on the separation of D,L-tryptophan were investigated.The retention times of the solutes decrease and the resolution increases with the increase of buffer pH from 6.0 to 8.0.With the increase of buffer concentration from 5 to 50 mmol/L,the retention times of the solutes increase,while the resolution increases to a higher-value and then decreases.The organic modifier is not conducive to the chiral separation in this experimental system.A best separation of tryptophan enantiomers is achieved by using pH 7.5,20 mmol/L phosphate buffer,with the resolution of 2.44.
【Fund】: 国家自然科学基金(20876128);; 福建省自然科学基金(E0610030)项目
【CateGory Index】: O657.8
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 YAN Li-Juan~(1,2), ZHANG Qing-He~(1,3*), ZHANG Wei-Bing~1, FENG Yu-Qi~2, ZHANG Li-Hua~1, LI Tong~(1,3), ZHANG Yu-Kui~1 (1. National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China; 3. Dalian Elite Analytical Instruments Co. Ltd., Dalian 116011, China);Preparation of Silica-bonded Bovine Serum Albumin Chiral Monolithic Column for Enantiomeric Separation by Capillary Electrochromatography[J];Chemical Research In Chinese Universities;2005-05
Chinese Journal Full-text Database 10 Hits
1 ZHANG Zhi-juan et al(College of Biotechnology and Food Science,Tianjin Commerce University,Key Laboratory of Food Biotechnology of Tianjin City,Tianjin 300134);Research on the Extraction of the Active Protein of Extracellular Penicillium TS67 with PEG/Phosphate Aqueous Two-phase System[J];Journal of Anhui Agricultural Sciences;2009-17
2 WU Shao-hui et al(College of Pharmacy,Dali University,Dali,Yunnan 671000);Separation of Antitumor Active Plypeptides from Periplaneta americana by Ultra Filtration Technology[J];Journal of Anhui Agricultural Sciences;2012-04
3 ZHENG Nan,ZHU Jian-hang,LIU Jie (College of Environmentel Science and Engineering,Nanchang University,Nanchang 330029,China);The Partition Properties of Bovine Serum Albumin in Polyethyleneglycol(PEG)/Dextran Aqueous Two-Phase System[J];Amino Acids & Biotic Resources;2006-03
4 LUAN Mei-li YUAN Qi-peng(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029,China);Study on optimum conditions of cell disruption of the Agrobacterium tumefaciens strain with antioxidant[J];Journal of Beijing University of Chemical Technology(Natural Science Edition);2006-05
5 WU Jun , AI Qi-Jun , YU Tong-Quan, LI Mei, ZHA Gang (Beijing Agricultural College, Beijing 102206);Membrane Choose for Ultraflltrating of Soybean Whey[J];Journal of Beijing Agricultural College;2003-03
6 HUANG Shuping,YAO Jinshui,ZHANG Xiyan,JIANG Wenqiang,HE Qianqian,WANG Yike(College of Material Science and Engineering,Shandong Institute of Light Industry,Jinan 250353);Recent Development of Chiral Stationary Phase Based on Amide[J];Materials Review;2007-08
7 WANG Shao jun 1,LI De cai 2,AN Qing da 1,WANG Yan 1,ZHAI Bin 1 ( 1.Dept. of Chem. Eng., Dalian Inst. of Light Ind., Dalian 116034,China; 2.Shenyang Parkerizing Co.,Ltd. Shenyang 110044, China );Preparation of nanoparticles SO_4~(2-)/ZrO_2 by reversed of microemulsion method[J];Journal of Dalian Institute of Light Inoustry;2001-04
8 SHANG Rui1,2,ZENG Hui-cai1,HaoJia2,3(1.State Key Biotechnology Laboratory for Tropical Crops,CATAS,Haikou 571101,China;2.College of Environment and Plant Protection,Hainan Uninversity,Danzhou 571737,China;3 Analysis and Testing Center,CATAS,Haikou,571101,China);Progress on the research in the agricultural antibiotics s eparation and purification[J];Guangdong Agricultural Sciences;2008-02
9 SUN Guoyong, SHI Qinghong, SUN Yan(Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072);PREPARATION OF A BIPOROUS POLYMERIC ADSORBENT BY DOUBLE EMULSIFICATION FOR FLOWTHROUGH CHROMATOGRAPHY OF PROTEINS[J];Acta Polymerica Sinica;2006-02
10 XU Rong-qiang,XU Huai-dong,YE Li (School of Chemical Engineering,Sichuan University,Chengdu 610065,China);Study on the Production of Ethanol with Membrane Technology[J];Filter & Separator;2003-02
China Proceedings of conference Full-text Database 2 Hits
1 Qiong Wu,Cheng Cai,Yuanchao Wang (College of Material,Chemistry and Chemical Engineering,Hangzhou Normal University);Preparation and performance of inorganic silica gel-organic polymer mixture based chiral porphyrin capillary monolithic column[A];[C];2011
2 YU Xianguo,YU Ziying,LI jing (Shanghai Dehong Biomedical Science and Technology Development Co.,Ltd.,Shanghai 200093,China);The opportunities and prospects for development of membrane science and technology[A];[C];2012
Chinese Journal Full-text Database 10 Hits
1 ZHANG Zhi-Sheng,YANG Yi~*(School of Science,Beijing University of Chemical Technology,Beijing 100029,China);Preparation and Evaluation of Open-tubular Capillary Electrochromato-graphy Column with the Poly(amidoamine)(PAMAM) Dendrimers as Bonded Stationary Phase[J];Chemical Journal of Chinese Universities;2006-01
2 GUO Bao-Jing,YANG Yi,SU Ping(College of Science,Beijing University of Chemical Technology,Beijing 100029,China);Preparation of Open-tubular Capillary Electrochromatography Column with the BSA-Poly(amidoamine)(PAMAM) Dendrimer as Bonded Stationary Phase[J];Chemical Journal of Chinese Universities;2007-07
3 ZHANG Yuzhong~1, 2 , BORNEMAN Z~2, KOOPS G-H~2, WESSLING M~2( ~1 Key Lab of Hollow Fibre Membrane Materials & Membrane Process(Tianjin Polytechnic University), Ministry of Education, Tianjin 300160)( ~2 Membrane Technology Group, Twente University, The Netherlands);STUDIES OF ADSORPTION OF BOVINE SERUM ALBUMIN ON RESIN MIXED PES FIBROUS ADSORBENTS[J];Acta Polymerica Sinica;2006-02
4 XU Dong-mei, NING Chun-hua, ZHANG Ke-da, ZHU Xiu-lin (Depart. of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215006, China);THE METHODS OF SYNTHESIZING AND CHARACTERIZING DENDRIMERS[J];Polymeric Materials Science & Engineering;2004-01
5 Chen Guangqiu, Guo Jianwei, Fan Chaosong, Hu Zhirong, Lu Jiajiu (Guangzhou Research & Development Development for Irradiation Technology, 511495) (Faculty of Chemical Engineering and Light Industry, GDUT, Guangzhou, 5l0090);Progress of Application of Microwave in Chemical Synthesis[J];Guang Zhou Chemical Industry and Technology;2001-02
6 Ni Chunmei Sheng Fengjun (Jiangsu Radio and TV University Yixing Academy 214206);The Application of the Microwave Technology on Chemical Synthesis[J];Guang Zhou Chemical Industry and Technology;2004-02
7 CHEN Jian-long~(1,2),QI Jian-cheng~1,CAO Yi-zhi~2,GUO Yong~1,DUAN Hui-li~1(1.Institute of Medical Equipment,Academy of Military Medical Sciences, National Engineering Research Center for Biological Protective Equipment,Tianjin 300161,China;2.School of Life Science,Lanzhou University,Lanzhou 730000,China);Research Progress of Immobilized Enzyme[J];Chemistry & Bioengineering;2006-02
8 HE Rui,CAO Guangqun,CHEN Mingqing,YANG Cheng,YANG Ji (School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,Jiangsu,China);Preparation of polystyrene microspheres with sulfonic acid group on the surface and adsorption of bovine serum albumin[J];Chemical Industry and Engineering Progress;2007-07
9 DENG Jun,LI Mingchun,XIN Meihua,GE Yafang(College of Material Science and Engineering,Huaqiao University,Key Laboratory of Functional Materials for Fujian Higher Education,Quanzhou 362021,Fujian,China);Adsorption of bovine serun albumin on chitosan microspheres modified by α-ketoglutaric acid[J];Chemical Industry and Engineering Progress;2008-12
10 SHA Yao wu, WU Jie (Chemistry Department of Tsinghua University, Beijing 100084, China);The Development and Potential Application of Dendrimer[J];HUNAN CHEMICAL INDUSTRY;2000-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Yan Lijuan 1,2,3, Zhang Lingyi 3, Zhang Qinghe 2, Li Tong 2, Feng Yuqi 1, Zhang Weibing 3, Zhang Yukui *3 1 (College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072) 2(Dalian Elite Analytical Instruments Co. Ltd., Dalian 116011) 3 (National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011);Preparation of Weak Anion-Exchange Silica Monolithic Column for Capillary Electrochromatography[J];Chinese Journal of Analytical Chemistry;2004-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved