Detection of Multidrug Resistance Gene in 51 Cases of Patients with Lung Cancers and its Clincal Significance
Dai Guanghai; Shi Tingzhang; Li Chunhai (General Hospital of PLA,Beijing, Beijing 1000853;Basic Institute of Asademy of Military Medical Sciences of PLA, Beijing 100850)
(Purpose) To study the expression of MDR1 gene in patients with lung cancer and its clincal significance. (Methods)Detection of multidrug resistance gene in 51 lung cancer specimens and corresponding normal lung tissue specmens from the neighbourhood of the cancer focuses by reverse transcription-polymerase chain reaction (RT- PCR ). These cases did not undergo any anti -cancer treatment. (Results) 7 of the 51 nortnal tissue(1 3. 7 % ) and 22 of the 51 tumor specimens(43. 1 % ) expressed MDR1 gene (P＜0. 01 ).No correlations were noted among MDR1 gene expression, histological differentiation, pathological type, and TNM stage.(Conclusions) These results suggest that the level of MDR1 gene expression in lung cells is incresaed as cells progress from the normal to the transformed state. So it might be a tumor marker of lung caner. Moreover, it is a importment factor of drug resistance mechanism of lung cancer.