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The Influence of Rac1shRNA on Invasive Ability of Ovarian Cancer Cell Skov3

WANG Hong1,2,ZHANG Ju-qing1,CUI Man-hua1,et al. (1. the Second Hospital of Jilin University,Changchun 130041,China;2. the Affiliated Hospital of Beihua University,Jilin 132011,China)  
[Purpose] To observe the influence of Rac1shRNA on invasive ability of ovarian cancer cell Skov3. [Methods]Rac1shRNA vector pGPU6/GFP/Rac1-524 mediated by liposome Lipofectamine TM 2000 was transfected to human ovarian cancer cell lines Skov3. Migration and invasion ability of transfected cell were detected by Transwell invasion test,and ultrastructural changes of Skov3 cells were observed by scanning electron microscopy.[Results]In pGPU6/GFP/Rac1-524 transfection group,the shortest migration distance of transfected cell,lamellipodia disappeared,invasive ability of transfected cell declined were found,those were significanty different between pGPU6/GFP/NC transfection group and the untransfection group. [Conclusion]Rac1shRNA vector pGPU6/GFP/Rac1-524 might effectively inhibit the migration and invasion of ovarian cancer cell Skov3 by making the cell pseudopod disappeared.
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