Detection of VEGF-C and VEGF-D mRNA in Cervical Cancer Early Stage and Sentinel Lymphatic Node by Real-Time PCR
ZHAO Qun,WU Yu-mei,WANG Jian-dong,et al. (Beijing Obstetics and Gynecologic Hospital,Capital Medical University,Beijing 100006,China)
[Purpose] To investigate VEGF-C and VEGF-D mRNA levels in early stage of cervical cancer and sentinel lymphatic nodes(SLN). [Methods] The cancer tissues,pericancerous tissues, positive SLN,negative non-SLN and normal tissues in 51 cases with cervical cancer early stage were collected. Lymphatic metastasis was observed using transmission electronic microscope, VEGF-C and VEGF-D mRNA were detected by real-time PCR,and the relationships of VEGF-C and VEGF-D mRNA with clinicopathologic factors were studied. [Results] With transmission electronic microscopy,there were hyperplastic lymphatic vessels surrounding the cervical cancer tissues. Expressions of VEGF-C and VEGF-D mRNA in cancer tissues,pericancerous tissues,or positive SLN tissues were significantly higher than those in negative non-SLN or normal tissues (P0.05). The expressions of VEGF-C and VEGF-D mRNA in cervical cancer were significantly different between those with and without lymph node metastasis,those with and without lymph vessel invasion,and depthes of tumor invasion (P0.05),but there was no significant difference between ages,stages,lesion sizes,grades,or pathologic types (P0.05). [Conclusion] VEGF-C and VEGF-D play an important role in lymph node metastasis of cervical cancer early stage. VEGF-C and VEGF-D may be crucial indexes for predicting lymph nodes metastasis,and may become treatment target for inhibition of lymph node metastasis of cervical cancer.