Expression of miR-29 and Its Relationship with Vascular Endothelial Growth Factor-A(VEGF-A) in Gastric Cancer
LI Xue-cheng1,LU Bing2,WANG Zong-hua3,et al. (1. No. 324 Hospital of PLA,Chongqing 400020,China; 2. Xinqiao Hospital,the Third Military Medical University,Chongqing 400038,China; 3. Nursing School,the Third Military Medical University,Chongqing 400038,China)
[Purpose] To investigate the expression of miR-29a and its relationship with vascular endothelial growth factor-A(VEGF-A) in gastric cancer tissues and serum. [Methods] The expression of serous miR-29a and VEGF-A in 50 cases with gastric cancer were detected by real-time PCR and ELISA,respectively. The expression of VEGF-A in 113 gastric cancer tissues (57 cases with miR-29a high expression,56 cases with miR-29a low expression) were detected immunohistochemically,and the expressions between serous and tissues miR-29a or VEGF-A in gastric cancer were analyzed. The miR-29a binding site on VEGF-A 3’UTR sequence was analyzed by bioinformatic predictions program. [Results] The expression of miR- 29a and VEGF-A in the serum of patients with gastric cancer was significantly negative correlation( R2=0.56,P0.0001). The expression of VEGF-A in low miR-29a expression group of gastric cancer tissues was significantly higher than that in high miR-29a expression group (74.32%±7.98% vs 34.37%±6.59% ,P 0.001). VEGF-A 3’UTR sequence might have stable binding site with miR-29a.[Conclusion] By suppressing VEGF-A expression,miR-29a is probably a potential tumor suppressing miRNA.