Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Xinjiang Agricultural Sciences》 2005-03
Add to Favorite Get Latest Update

A simple and quick method for DNA isolation in sugarbeet

SHA Hong~1,WANG Yan-fei~1,QU Yan-ying~2,GAO Wen-wei~2,ZHANG Li-ming~1,LIU Jun~1 (1.Institute of Industrial Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830000,China;2.College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China)  
Sugarbeet DNA was extracted from leaves of sugarbeet with three different methods CTAB,SDSⅠand SDSⅡ.The DNA was examined with ratios of OD_(260) /OD_(280), concentration and PCR application. The results of experiments showed that the DNA concentration with CTAB is high,the best pure. So the CTAB is the way to extract DNA that can be used in molecular marker study.
【Fund】: 自治区高技术发展计划项目"甜菜重要农艺性状及离子注入诱变性状的分子标记辅助育种"(200415114)
【CateGory Index】: S566.3
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved