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《Xinjiang Agricultural Sciences》 2010-01
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Establishment of Double Direction Gel Electrophoresis of Cotton Fibre Protein

WANG Juan1,2,NI Zhi-yong1,SHI Qin-hua2,Lü Meng1,2,LI Bo1,2,BAI Yan1,FAN Ling1(1.Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;2.College of Agronomy,Xinjiang Agricultural University,Urumqi 830052,China)  
【Objective and Method】The objective of this paper was to compare with three different protein extraction methods of Gossypium hirsutum,cv.Xuzhou 142 and the effects of various lysis buffers on the double direction electrophoresis(2-D).The loading quantity of sample and staining method were also optimized for the 2-D analysis.【Result】The results showed that the protein content of cotton fibre extracted by saturated powder was highter than that extraced by other methods and the electrophoresis strip of SDS-PAGE were clear;the solubility raised whose protein were dissolved in the mixture of thiourea and CHAPS;the more suitable loading quantity was 300 μg to the pH 3-10 7 cm IPG strip.Furthermore,the protein spots were increased and the background was clear by the modified Coomassie Brilliant Blue method.【Conclusion】The double direction Gel Electrophoresis method used to extract cotton fibre protein was initially established.
【Fund】: 国家自然科学基金项目(30660088);; 国家“863”项目(2006AA10Z184);; 新疆维吾尔自治区高技术研究发展计划项目(200611101);; 农业部转基因重大专项课题(2009ZX08005-011B)
【CateGory Index】: S562.035.2
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