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《Xinjiang Agricultural Sciences》 2017-02
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Silencing Effect of Jasmonic Acid Carboxyl Methyl Transferase (JMT) Gene on Physiological Indexes of Cotton Seedlings

AN Wen-kai;YAN Xiao-hong;BAO Qiu-juan;ZHANG Fu-chun;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University;  
【Objective】Jasmonic methylester transferase( JMT) can effectively facilitate the production of jasmonic methylester,so that it enhances plant drought resistance.【Method】A 450 bp silence fragment of Gh JMT was cloned from cotton XLZ17( Gossypium hirsutum) using PCR amplification,and then constructed into vector p TRV. The recombinant plasmid p TRV- Gh JMT was transformed into competent Agrobacterium tumefaciens( GV3101). Cotton seedlings in hydroponic conditions were infected with the transformed A. tumefaciens GV3101. After one month,the growing cotton seedlings were treated with PEG6000 for 24 h. The water content,the molality of osmotic adjustment substances,photosynthetic pigments,antioxidants and malondialdehyde( MDA) as well as enzymes activities in cotton leaves under drought stress were measured.【Result】The results showed that content of soluble sugar,ASA,betaine,proline and SOD were reduced by 42. 1%,36. 6%,69. 4% and 71. 6% compared with the p TRV- 00,and MDA,O2-and H2O2 were increased 0. 43,1. 45 and 0. 59 times compared with p TRV- Gh JMT,respectively.【Conclusion】Our results demonstrate that Gh JMT gene silencing can directly affect the physiological and biochemical characteristics of cotton seedlings treated with drought stress and reduce drought resistant ability of cotton.
【Fund】: 国家自然科学基金-新疆联合基金重点项目“棉花水分高效利用的关键基因的挖掘及分子机理的研究”(U1303282)~~
【CateGory Index】: S562
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【Citations】
Chinese Journal Full-text Database 10 Hits
1 An Wenkai;Chang Dan;Zhang Fuchun;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University;;Improving the Physiological Response of Cotton by Spraying 2,4-Epibrassinolide(EBR) under the Drought[J];基因组学与应用生物学;2015-10
2 AN Wenkai;CHANG Dan;ZHANG Fuchun;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University;;Expression Characteristics of Transcription Factor BES1/BRI1 of Cotton Seedling in Response to Brassinosteroid under Drought Stress[J];西北植物学报;2015-07
3 CHANG Dan;YANG Yi;WANG Yan;ZHANG Xueyan;ZHANG Fuchun;LI Fuguang;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University;Cotton Research Institute,Chinese Academy of Agriculture Sciences;;Effects of 24-Epi Brassinolide on Seed Germination under Stresses of Salt and PEG in Cotton[J];西北农业学报;2015-03
4 Wang Li;Mu Chun;Zhang Mingcai;Du Mingwei;Tian Xiaoli;Li Zhaohu;Engineering Research Center of Plant Growth Regulator,Ministry of Education/College of Agronomy and Biotechnology,China Agricultural University;College of Life Science,Henan Normal University;;Effect of Silencing GhCPS on the Growth and Endogenous Hormone Content of Cotton Seedlings(Gossypium hirsutum L.)[J];棉花学报;2014-03
5 ZHOU Shouhua;LIU Zhongli;HAN Huanqing;WANG Xiang;FANG Wen;Jingzhou Agrometeorological Experiment Station of Hubei Province;Bortala Mongol Autonomous Prefecture Meteorological Bureau;;Influence Analysis of Meteorological Condition on Cotton Yield in Bortala Mongol Autonomous Prefecture of Xinjiang[J];沙漠与绿洲气象;2014-02
6 Muherepiya Aierken,ZHANG Fu-chun (Xinjiang Key Laboratory of Biological Resources and Genetic Engineering/College of Life Science and Technology,Xinjiang University,Urumqi 830046,China);Physiological and Biochemical Analysis for the Salt Tolerance of Transgenic Cotton Transformed by the Vacuolar H~+-Pyrophosphatase Gene(VP1) from Halostachys caspica[J];新疆农业科学;2013-06
7 Chen Liping He Daoyi School of Life Sciences,Huaibei Normal University,Anhui Key Laboratory of Resources and Plant Biology,Huaibei,235000;Research Advance on Drought and Salt Resistant Genes in Transgenic Plants[J];基因组学与应用生物学;2010-03
8 Li Qinging Li Dapeng Li Dequan(National Key Laboratory for Crop Biology,College of Life Science,Shandong Agricultural University,Taian 271018);The Research Progress in Biosynthesis and Regulation of Jasmonates[J];生物技术通报;2010-01
9 DONG Taoxing,CAI Kunzheng,ZHANG Jingxin,RONG Hui,XIE Guozheng,ZENG Rensen Institute of Tropical and Subtropical Ecology,South China Agricultural University,Guangzhou 510642,China;The physiological roles of methyl jasmonate (MeJA) in drought resistance of rice seedlings[J];生态环境;2007-04
10 ZHU Jia-hong,PENG Shi-qing~*(State Key Laboratory of Tropical Crop Biotechnology,Institute of Tropical Biosciences and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China);Jasmonic Acid and Its Signal Transmission[J];西北植物学报;2006-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Li Shuye;Ma Huijuan;Zhang Siping;Liu Shaodong;Shen Qian;Chen Jing;Ge Changwei;Pang Chaoyou;Zhao Xinhua;Institute of Cotton Research of Chinese Academy of Agricultural Sciences/State Key Laboratory of Cotton Biology;;Effects of Exogenous 24-epibrassinolide on Photosynthetic Physiology of Cotton-Seedlings under Low Temperature[J];棉花学报;2018-03
2 Qi WU;Pingsheng LENG;Zenghui HU;College of Landscape Architecture,Beijing University of Agriculture;Beijing Collaborative Innovation Center for Eco-environmental Improvement with Forestry and Fruit Trees;;Cloning and Expression Analysis of Allene Oxide Synthase Gene Ls AOS in Lilium ‘Siberia'[J];Agricultural Biotechnology;2018-02
3 Wu Qi;;Effect of methyl jasmonate(MeJA) on floral scent of Lilium “Siberia”[J];江苏农业科学;2018-06
4 Yao Yanli;Hu Xiaowen;Xu Lei;Xing Shulian;Gao Yuyao;An Dongsheng;Liu Yang;Guangdong Engineering Technology Research Center for Dryland Water-saving Agriculture, Zhanjiang Experiment Station, Chinese Academy of Tropical Agricultural Sciences;;Cloning and Expression Analysis of Ssδ-OAT-2 Gene from Saccharum spontaneum L.[J];分子植物育种;2018-06
5 Lei Ning;Li Shuxia;Peng Ming;College of Tropical Agriculture and Forestry, Hainan University;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Agriculture Sciences;;Cloning and Expression Analysis of MeTCP4 Transcription Factor from Cassava and Construction of Plant Expression Vector[J];分子植物育种;2018-05
6 Zheng Bingfeng;Ma Yuzhu;Lu Huadan;Sun Sen;An Wenkai;Zhang Fuchun;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University;;Effects of Spraying Brassinolide on Expression of Steroid 5~-Alpha Reductase Gene(Gh DET2) in Cotton under Drought Stress[J];基因组学与应用生物学;2018-02
7 Li Baoyan;Wang Yingzi;Jiang Faxiang;Shi Jie;Liu Xueqing;Yantai Academy of Agricultural Sciences;;Study on Physiological and Biochemical Defense Responses of Grape Against Infection of Plasmopara viticola[J];山东农业科学;2018-01
8 Jiang Maoshuang;Yuan Xiangmei;Liu Xiaodong;Hou Rui;Su Yanbing;Guo Zhan;Yu Shihui;Li Hongying;College of Agriculture,Shanxi Agricultural University;Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops;Institute of crop science,Shanxi Academy of Agricultural Sciences;Urban Agricultural Committee of Shanxi Jincheng;;Analysis of oleosingene family and its response to drought in foxtail millet[J];山西农业大学学报(自然科学版);2018-01
9 NIE Wen-jing;WANG Shuo-shuo;JING Xin;GONG Biao;WEI Min;YANG Feng-juan;LI Yan;SHI Qing-hua;College of Horticulture Science and Engineering,Shandong Agricultural University/Stage Key Laboratory of Crop Biology/Ministry of Agriculture Key Laboratory of Horticultural Crop Biology and Germplasm Creation in Huang-Huai-Hai region;;Effects of exogenous 2,4-epibrassinolide on the growth and redox balance of cucumber seedlings under NaHCO_3 stress[J];应用生态学报;2018-03
10 ZHOU Ziwei;CHANG Xiaojun;YOU Fangning;DENG Tingting;SUN Yun;LAI Zhongxiong;Key Laboratory of Tea Science in Fujian Provincy,College of Horticulture,Fujian Agriculture and Forestry University;Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University;;Analysis of Molecular Evolution and Codon Bias of Lipoxygenase( LOX) Gene Family in Tea Tree[J];中国农业科技导报;2017-12
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 CHANG Dan;ZHANG Xia;ZHANG Fuchun;Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University;;Cloning and Expression Analysis of HcPEAMT Gene from Halostachys caspica[J];西北植物学报;2014-08
2 GUO Hui-qin;REN Wei-bo;LI Ping;WU Zi-nian;WAN Dong-li;College of Life Sciences,Inner Mongolia Agricultural University;Institute of Grassland Research of Chinese Academy of Agricultural Sciences;;Effect of epi-brassinosteroid and Gibberellin on seed germination and seedling growth of Leymus chinensis[J];草业科学;2014-06
3 XIONG Jun-lan;KONG Hai-yan;BAI Xue;TAN Rui-yue;XIONG You-cai;CHEN Hong-bin;School of Life Sciences,State Key Laboratory of Grassland Agro-ecosystems,Lanzhou University;Center of Urban Ecology and Environmental Biotechnology,Lanzhou City University;;Efects of brassinosteroids on plant drought adaptability and their regulatory mechanism[J];兰州大学学报(自然科学版);2013-05
4 WU Xue-Xia;ZHA Ding-Shi;ZHU Zong-Wen;XU Shuang;Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Protected Horticultural Technology;;Effects of Exogenous 24-Epibrassinolide on Plant Growth and Antioxidant System in Eggplant Seedlings under High Temperature Stress[J];植物生理学报;2013-09
5 QIAN Wen-jing;LIU Jing;SONG Dan;HE Zheng-quan;Biotechnology Research Center of China Three Gorges University;;Research Progress on Brassinosteroid Related Genes and Their Functions in Rice[J];河南农业科学;2013-09
6 Muherepiya Aierken,ZHANG Fu-chun (Xinjiang Key Laboratory of Biological Resources and Genetic Engineering/College of Life Science and Technology,Xinjiang University,Urumqi 830046,China);Physiological and Biochemical Analysis for the Salt Tolerance of Transgenic Cotton Transformed by the Vacuolar H~+-Pyrophosphatase Gene(VP1) from Halostachys caspica[J];新疆农业科学;2013-06
7 HUANG Jiujun1,SHI Guoqiang1,DENG Xiaoxia2(1.Qiemo Meterological Bureau,Qiemo 841900,China;2.College of Agriculture,Shihezi University,Shihezi 832003,China);Characteristics of Frost Change and Its Influence on Main Crops in Qiemo Oasis During 1961-2010[J];沙漠与绿洲气象;2013-02
8 HUANG Gang,DU Zhenzhu,CAO Ting,ZHANG Hui and YAN Ping(College of Life Science,Shihezi University,Shihezi Xinjiang 832003,China);Seed Germinating Characteristics of Betula microphylla[J];西北农业学报;2013-04
9 XU Fen-fen,XU Wei-hong(College of Life Science,Shangrao Normal University,Shangrao 334001,China);Induction of Resistance to Downy Mildew in Brassica campestris ssp. chinensis L. by Brassinolide[J];河南农业科学;2012-12
10 YAN Cai-hong1,LI Xiao-ming2(1.Wulanwusu Agrometeorological Experiment Station,Shihezi,832003,China; 2.Graduate University of Chinese Academy of Sciences,Beijing 100049,China);Meteorological Factors Influencing Cotton Yield in Xinjiang Based on Gray Conjunction Analysis[J];沙漠与绿洲气象;2012-05
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