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《Xinjiang Agricultural Sciences》 2017-07
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Identification of the Mutator Insertional Mutants in ZmCIPK23

CHEN Guo;CHEN Xun-ji;LI Jian-ping;HAO Xiao-yan;CHANG Xiao-chun;Zumuremu;ZHENG Jun;HUANG Quan-sheng;Research Institute of Nuclear & Biotechnology,Xinjiang Academy of Agricultural Sciences;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences;  
【Objective】The candidate mutants of maize ZmCIPK23 gene were continuously backcrossed and self-pollinated with B73 in fields,and finally homozygous mutants with B73 background were obtained,which laid the material foundation for studying the function of this gene.【Method】The candidate mutants of ZmCIPK23 were screened from a maize Mutant library.They were planted and analyzed by nested PCR.The positive plants with Mu-insertion in the ZmCIPK23 gene were self-pollinated and backcrossed with B73 in the field.Thus the homozygous mutants were obtained.【Result】The homozygous mutant of ZmCIPK23 gene was obtained by PCR.【Conclusion】The homozygous mutant( zmcipk23) of Mu insertion of ZmCIPK23 gene was obtained.This study has provided a foundation for the functional analysis of the ZmCIPK23 gene.
【Fund】: 新疆维吾尔自治区自然科学基金“玉米干旱胁迫相关基因Mu插入突变体的鉴定及抗旱性”(2013211B22)~~
【CateGory Index】: S513
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