Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Xinjiang Agricultural Sciences》 2018-01
Add to Favorite Get Latest Update

Screening and Identification of Cellulolytic Strains from Cotton Straw and Its Effect on Degradation of Cotton Stalk

SUN Mei-na;ZHANG Fan-fan;WANG Yong-qiang;WEI Xiao-yan;LU Jian-jiang;School of Chemistry and Chemical Engineering,Shihezi University/Key Laboratory for Green Processing of Chemical Engineering,Xinjiang Production and Construction Corps;College of Animal Science and Technology,Shihezi University;College of Animal Science and Veterinary Medicine,Henan Institute of Science and Technology;Wuwei Academy of Animal Husbandry and Veterinary Sciences;  
【Objective】This study aims to screen and identify fungi which adhere on the surface of the cotton by using microbiology and molecular biology methods in order to obtain fungi with high cellulose decomposition ability.【Method】The cotton straw was inoculated into different selective media,and the larger hydrolysis circle of strains was separated and selected. At the same time the strains' filter paper enzyme activity( FPA) and sodium carboxymethyl cellulose( CMC-Na) saccharification vitality were determined,and besides,the weight loss rate of cotton stalk fiber was observed continuously for 25 days.【Result】The diameter of the hydrolysis ring of the 4 main strains was SN-1 SN-4 SN-3 SN-4( P = 0. 013). In terms of CMC-Na saccharification vitality,SN-1 was significantly lower than other three strains( P 0. 05) at24-96 h,and there was no significant difference between different strains of treatments( P 0. 05) at 120 h. In terms of FPA activity,SN and SN 1-4 strains was significantly higher than SN-2 and SN-3 strains( P 0. 05) at 24 and 120 h,at other time points,the strains were sorted according to the activity level: SN-1,SN-4 SN-3 SN-2( P 0. 05). There were 4 bacterial strains obtained by using the method of microscopic and molecular identification,SN-1 was Aspergillus nige r,SN-2 was Gibberella moniliformis,SN-3 was Alternaria nees and SN-4 was Penicillium italicum. 【Conclusion】The effect of Aspergillus niger on the degradation of cotton straw was the most remarkable. The activity of CMC-Na saccharifying enzyme,the activity of filter paper enzyme and the actual weight loss rate( degradation rate) of cotton straw were the best when inoculated with Aspergillus niger or Penicillium italicum.
【Fund】: 兵团科技援疆项目(2013AB008);; 新疆维吾尔自治区研究生科研创新项目(XJGR2015037)~~
【CateGory Index】: S141.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved