Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Xinjiang Agricultural Sciences》 2018-01
Add to Favorite Get Latest Update

Optimization of Fermentation Medium Components for Dihydrodaidzein Producing Strain Using RSM

XIE Jing-long;LI Xiao-bin;ZHAO Fang;WANG Chen-chen;YANG Kai-lun;Xinjiang Key Laboratory of Herbivore's Nutrition for Meat and Milk Production;  
【Objective】he aim of this experiment is to optimize the main nutrient components of the fermentation medium of dihydrodaidzein producing strain by single factor test design and response surface methodology.【Method】On the basic of BHI medium,the main factors( carbon source,nitrogen source,growth factor and inorganic salt) and the appropriate range of concentration were determined by single factor test. The best composition in the medium was analyzed by the Box-Behnken central composite experiment and response surface methodology.【Result】The significant factors such as glucose,peptone,VB_1 and KH_2PO_4 were determined by the single factor experiment and response surface methodology analysis was determined that the supplemental doses to the strain medium were 10. 22 g/L glucose,6. 00 g/L peptone,0. 49 g/L VB_1 and 0. 82 g/L KH_2PO_4 on the basic of BHI medium. Validation experiments showed that the DHD concentration was 0. 27μg/m L,which was close to the predictive value 0. 28 μg/m L.【Conclusion】The results from the single factor experiment and response surface methodology showed that the optimal ingredients of medium for DHD production were 38 g/L BHI,10. 22 g/L glucose,6. 00 g/L peptone,0. 49 g/L VB_1 and 0. 82 g/L KH_2PO_4.
【Fund】: 新疆维吾尔自治区重点实验室开放课题“雌马酚高效转化细菌筛选及富含雌马酚牛乳生产的研究”(2016D03012)~~
【CateGory Index】: TQ920.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved