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EFFECTS OF GROWTH FACTORS ON THE IN VITRO GROWTH OF HIRCINE NON ANTRAL FOLLICLE OOCYTES

Zhou Huanmin 1,Zhang Yong 2 (1 Department of Animal Science,Inner Mongolia Institute of Agriculture and Animal Scinece,Huhhot 010018; 2 Research Department of Developmental Biology, Northwestern Agricultural University,Yangling,71  
WT5BZ]In the presence of FSH,the hircine non antral follicle oocytes were cultured for 9 days with IGF I,EGF and bFGF alone or combinedly.The results showed that IGF I(100ng/mL)effectively maitained the survival of follicle oocytes and promoted their growth,and the survival rate and the relative growth rate of oocytes were 75 00% and 45 06% respectively.EGF(50ng/mL)enhanced the survival rate of the oocytes like IGF I,but had a negative effect on the growth of oocytes.bFGF(50ng/mL)had a promoting effect on the survival in vitro of oocytes but not obvious on the growth of them (the survival rate and the relative growth rate of oocytes were 76 92% and 31 55% respectively).IGF I(100ng/mL) and EGF(50ng/mL)combination had the highest effects on both of the survival and the growth of oocytes.The survival rate and the relative growth rate of oocytes were 90 91% and 48 24% respectively.It has been shown that in the presence of FSH these kinds of growth factors alone or choosingly combinedly gave a positive regulation to the survival in vitro of hircine non antral follicle oocytes.
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