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THE PREPARATION OF SWINE PSEUDORABIES OIL EMULSION INACTIVATED VACCINE AND EXPERIMENTS ON ITS SAFETY AND IMMUNITY

Chen Huanchun,Jin Meilin,He Qigai,Fang Liurong, Wu Meizhou,Zhou Bin,Zhou Rui (Animal Science and Veterinary Medicine College, Huazhong Agricultural University,Wuhan 430070)  
The virulent pseudorabies virus Ea strain which was isolated and identified by our lab,was cultivated on BHK 21 cell and used as vaccine antigen,The virus titer was over 10 6 TCID 50 /0 1 ml The vaccines were prepared four times following the procedure;i e,inactivation of virus with a certain concentration of Formal in and then emulsion of the killed virus with oil adjuvant In this paper the safety and immunity of the inactivated vaccine were reported The 18 gram weight mice were injected with 0 3 ml of vaccine,while newborn piglets,post weaning piglets and pregnant sows accepted as much two fold as normal dosage The fact that no any reverse reaction was observed and the sow′s production was not affected revealed the high safety of the vaccine The serum neutralization index of replacement gilts and pregnant sows were over 316 at 21st days post immunization At interval of 35 days the sows accepted another injection,the SN index could raise to over 10 000 After challenge with virulent pseudorabies virus,the protective rate of immunized post weaning and newborn piglets were 100% and 90 62%,respectively
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