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SEQUENCE ANALYSIS OF MAJOR PROTECTIVE ANTIGEN E2(gp55)GENE OF CHINESE HOG CHOLERA LAPINISED VIRUS DIRECTLY FROM RABBIT SPLEEN TISSUE(C STRAIN)

Han Xueqing,Li Hongwei,Liu Xiangtao,Li Xiaobing,Ma Junwu,et al. (Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046)  
The major protective antigen E2 gene of Chinese Hog Cholera Lapinised Virus(C strain)directly from rabbit spleen tissue,was amplified by reverse transcription (RT) and nested polymerase chain reaction(N PCR) and successfully cloned,it′s sequence was determined and compared with those of other virulent strains of HCV published natively or abroad.The result showed that,the nucleotide homologies of E2 gene of C strain directly from rabbit spleen tissue with C strain(adapted to SK6 cell),C strain(adapted to calf testicle cell,HCLV C),HCV SM strain(virulent strain Shimen),virulent strain Brescia(Netherlands)and virulent strain Alfort(Germany),were 98 87%,98 34%,94 58%,91 00% and 80 78% respectively.The amino acid homologies were respectively 98 95%,97 37%,94 22%,91 60% and 89 23%.In three neutralized antigenic domains A、B、C of E2 gene compared with the strain described aboved and epidemic virulent strain,there was very little difference or even no difference between C strain from rabbit spleen and C strain adapted to cells,while the difference between C strain form rabbit spleen and epidemic virulent strain,classic virulent strain in B、C domains was great.Morever compared with chinese classic virulent strain Shimen,there was great difference between our countrys′ virulent strains in 1980s and those in 1990s,this showed that the epidemic virulent strains of hog cholera virus have changed in our country.
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