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CLONING AND SEQUENCE ANALYSIS OF THE MAJOR OUTER MEMBRANE PROTEIN GENE OF AN OVINE ABORTION STRAIN OF CHLAMYDIA PSITTACI

Miao Zhenchuan 1,Hasi Agula 2,Zhao Yafang 1,Zhang Baofa 1,Zhang Heling 2 (1.Inner Mongolian Academy of Animal Science,Huhhot 010030; 2.Biotechnology Center of Inner Mongolian University,Huhhot 010021)  
In order to clone the major outer membrane protein(MOMP) gene of a goat abortion strain of Chlamydia psittaci isolated from Inner Mongolia area,a pair of primers were synthesized according to the published sequences of Chlamydial MOMP gene,and the genomic DNA was extracted from the purified Chlamydial particles and employed as templat,then polymerase chain reaction was performed.As a result,a 1.17 Kb DNA fragment was amplified.The PCR product was recovered from low melting point agarose gel and underwent cleavage by restriction enzymes whose recognition sites had been designed precedingly on the ends of the primers,then ligated to the corresponding site of plasmid pUC19.The ligation reaction mixture was used to trans form E.coli DH5α.The recombinant was screened by PCR detection or restriction enzymes analysis.The DNA sequencing was performed.It showed that this recombinant fragment covered the whole coding region of the MOMP gene whice was 1170 bp long.It also revealed that this MOMP gene of Chlamydial strain was just the same as that of England ovine abortion strain S26/3,and there was only one nucleotide synonymous mutation in comparison with that of strain B577.
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