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《Chinese Journal of Animal and Veterinary Sciences》 2002-06
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Cloning and Expression of Nucleoprotein Gene of Transmissible Gastroenteritis Virus

TANG Li jie,LI Yi jing,JIA Yong qing,WANG Jun wei,LIU Bao quan (Animal Medicine College of Northest Agricultural University,Harbin 150030,China)  
The viral subgenome mRNA of porcine transmissible gastroenteristis virus(TGEV)was amplified by RT PCR using primer pairs Li01 and Li02 which designed according to the reported reference.A DNA fragment was amplified which contains completely ORF gene and the size is about 1174bp and then the DNA fragement was cloned site specially into pProEXHTb expression vector between the NspV and XhoI cleavage site.The recombinant plasmid(PHN)was verified by restriction endonuclease analysis and nucleotide sequencing.Then it was transformed into E.coli strain TG1 for N gene expression.The expression product was identified by SDS PAGE and Western blot test,a fusion protein about 47KD as we expected was found.The results shows that the vitro expressed protein of N gene via recombinant plasmid vector in the E.coli maintains antigenicity of TGEV.
【Fund】: 黑龙江省科委“九五”攻关项目 (G99B8- 1- 1)
【CateGory Index】: S852.65
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