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《Chinese Journal of Animal and Veterinary Sciences》 2004-01
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Establish the Cultivation of Swine Vascular Endothelial Cell in Vitro

MU Yang,ZHANG Yan-ming(College of Animal Science-Technology,Northwest Science-Technology University ofAgriculture and Forestry,Yangling 712100,China)  
The present experiment studied the culture methods of swine vascular endothelial cell(SVEC)in vitro.The results were as follows:The inner membrane of vascular digested by collagenase I or trypsin could provide enough dispersed cells for primary culture.But the effect of collagenase Ⅰ was apparently better than that of trypsin.Fibroblasts sometimes mixing in the primary and early passage culture could be cleared away effectively by using lemon acid trypsin.On the condition of no extracellular matrix and endothelial cell growth factor,endothelial cell was successfully cultured by adding insulin to EC medium.The culture cells are observed with microscopy phase contrast and the cells arrange in shape of typical"cobble-stone"with contact inhibition in existing among cells.Factor Ⅷ-related antigen antibody indirect fluorescence staining can examine the obvious yellow-green fluorescent light in plasma around nucleus.All those can prove the cultured cell are endothelial cell.
【Fund】: 西北农林科技大学专项基金重点项目(2000 31)
【CateGory Index】: S852.2
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