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《Chinese Journal of Animal and Veterinary Sciences》 2004-01
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Establish the Cultivation of Swine Vascular Endothelial Cell in Vitro

MU Yang,ZHANG Yan-ming(College of Animal Science-Technology,Northwest Science-Technology University ofAgriculture and Forestry,Yangling 712100,China)  
The present experiment studied the culture methods of swine vascular endothelial cell(SVEC)in vitro.The results were as follows:The inner membrane of vascular digested by collagenase I or trypsin could provide enough dispersed cells for primary culture.But the effect of collagenase Ⅰ was apparently better than that of trypsin.Fibroblasts sometimes mixing in the primary and early passage culture could be cleared away effectively by using lemon acid trypsin.On the condition of no extracellular matrix and endothelial cell growth factor,endothelial cell was successfully cultured by adding insulin to EC medium.The culture cells are observed with microscopy phase contrast and the cells arrange in shape of typical"cobble-stone"with contact inhibition in existing among cells.Factor Ⅷ-related antigen antibody indirect fluorescence staining can examine the obvious yellow-green fluorescent light in plasma around nucleus.All those can prove the cultured cell are endothelial cell.
【Fund】: 西北农林科技大学专项基金重点项目(2000 31)
【CateGory Index】: S852.2
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3 SUN Guang-li 1,2,CAI Xue-hui 3,ZHENG Shi-min1,SUN Gang2,RONG Fu-long1 (1.College of Veterinary Medicine,Northeast Agricultural University,Harbin150030,China;2.Institute of Animal Health Inspection of Heilongjiang Province,Harbin150069,China;3.Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Harbin150001,China);Experimental studies on the culture of porcine reproductive and respiratory syndrome virus in porcine arterial endothelial cells[J];Chinese Veterinary Science;2010-10
Chinese Journal Full-text Database 2 Hits
1 Sun Jihu, Wang Yang , Yu Yanzhen, Jian Guowei, Wang Keqian (Department of Anatomy, Shanghai Medical Univeristy , Shanghai 200032);CULTURE AND MORPHOLOGY OF VASCULAR ENDOTHELIAL CELLS[J];Chinese Journal of Anatomy;1996-03
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1 ZHU Qi-xing1,2, MA Tai2, TU Deng-yun2, SHEN Tong2, DING Rui2(1.The First Affiliated Hospital of Anhui Medical University,Hefei 230031,Anhui,China; 2.Central Laboratory of Toxicology,Public Health School, Anhui Medical University, Hefei 230032,Anhui,China);Effect of Ginkgo Biloba Extract on Trichloroethylene-induced Nitric Oxide Synthesis in Human Keratinocytes[J];Carcinogenesis,Teratogenesis & Mutagenesis;2006-06
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4 LI Chun-xiang(Tangshan Teachers College,Tangshan,Hebei 063000);Study on Effects of Syngnthus.acus Linnaeus Extracts on Human Lymphocytes in Vitro[J];Journal of Anhui Agricultural Sciences;2009-03
5 WU Juan et al(Department of Pathology,College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018);Primary Culture of Bovine Mammary Epithelial Cells[J];Journal of Anhui Agricultural Sciences;2009-06
6 Wang Tong, Mei Xiaodong, Xu Xiaoling, et alDepartment of Respiratory Medicine, Hefei First Hospital,Hefei 230061;Establishment of multidrug-resistance lung adenocarcinoma cell line A_(549)/ADM and some characteristic[J];;2006-01
7 FAN Guo-chang, WU Chu-tse, WANG Yan-fei, et al. (Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China);Effect of human wild-type p53, GM-CSF and B7-1 genes on biological behavior of chemotherapy - resistant tumor cells via recombinant adenovirus vector[J];CHINESE JOURNAL OF CANCER;2000-01
8 JIN Ge 1 ,ZHANG Ting 2 ,WANG Tao 3 ,YANG Li-ping 2 1.Department of Biochemistry,Medi cal College,Zhengzhou University,Zhengzhou 450052,P.R.China.2.Department of Biochemistry,Henan Traditional Chinese Medical Colle ge,Zhengzhou 450003,P.R.China.3.Departmen;Inhibition of α-Interferon and Cinnamic Acid on Pro liferation of Human Lung Cancer Cell[J];Chinese Journal of Cancer;2002-08
9 Yue Tao1, Zhang Ping2, Liu Peng1, Deng Qing Li1, Ji Qiong Mei1, Li Xiu Ying1, Zhu Zhen Yu11.Department of Biochemistry,SunYat sen Medical College,Sun Yat senUniversity,Guangzhou,Guangdong,510080,P.R.China; 2.Sun Yat sen Ophthalmic Center,Sun Yat sen University,Guangzhou,Guangdong,510080,P.R.China;Effect of Transfection of hEndostatin Gene on CNE2 Cell Xenograft Growth in Nude Mice[J];Chinese Journal of Cancer;2003-02
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China Proceedings of conference Full-text Database 3 Hits
1 LU Luo, YING Kang, WEI Shaoming, LIN Huifen,MA Laiji,MAO Yumin Research & Development Department of Shanghai Jahwa Co. LTD., Shanghai 200082. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433.;The induced activity of asiaticoside for the dermal fibroblasts in collagen synthesis[A];[C];2004
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Chinese Journal Full-text Database 10 Hits
1 Man Guotong, Feng Zhihui, Zhang Tonglin, Zhou Xiaosi (The third hospital of beijing university, Department of general surgery, beijing 100083 China) \;;IMPROVEMENT IN THE TECHNIQUES OF CULTURING AND PURIFYING PIG, HUMAN, AND RAT ENDTHELIAL CELLS AND SMOOTH MUSCLE CELLS[J];Chinese Journal of Histochemistry and Cytochemistry;2001-02
2 LI Chun-mei,DENG Yu-xiu,WANG Dong-dong,SU Run-huan,ZHAO Li-chan,SONG Yan-hua(Guangdong Win's Group Academy,Xinxing 527400,China);Study on Growth Characterization of Different PRRSV Strains in Marc-145 Cells[J];China Animal Husbandry & Veterinary Medicine;2010-03
3 YU Yi-ke1,2,HU Jian-en1,BAI Xue-fang1,DU Yu-guang1,Lin Bing-cheng1(1.Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;2.Graduate University of Chinese Academy of Sciences,Beijing,100049,China;);Preliminary Study on Biological Activities of Globin Hydrolysate[J];Food and Drug;2006-08
4 HONG Hai-xia,ZHANG Yan-ming,SUN Pei,SU Zheng-yuan,WEI Zhong-feng,ZHANG Yong-guo (College of Animal Science and Technology,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Segregation and culture of endothelial cells from swine umbilicus veins[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry;2004-05
5 MA Quan-li,HONG Hai-xia,ZHANG Yan-ming,XU Hao,YANG Meng,SU Zheng-yuan(College of Animal Science and Technology,Northwest A & F Univercity,Yangling,Shaanxi 712100,China);Study on immortalization of swine umbilicus veins endothelial cells[J];Journal of Northwest A & F University(Natural Science Edition);2007-07
6 MU Yang, ZHANG Yan ming(College of Animal Science and Technology, Northwest Sci Tech University of Agriculture and Forestry, Yangling 712100, China);Study on growth characteristics of swine vascular endothelial cell in vitro[J];Chinese Journal of Veterinary Science and Technology;2003-05
7 WEI Tian-chao1,3,TIAN Zhi-jun1,AN Tong-qing1,ZHOU Yan-jun1,XIAO Yan1,JIANG Yi-feng1,HAO Xiao-fang1,ZHANG Shan-rui1,PENG Jin-mei1,QIU Hua-ji1,TONG Guang-zhi1,2*(1.Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences,Harbin 150001,China;2.Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200232,China;3.College of Animal Science and Technology,Guangxi University,Nanning 530005,China);Development and application of Taq Man-MGB fluorescence quantitative RT-PCR assay for detection of porcine reproductive and respiratory syndrome virus[J];Chinese Journal of Preventive Veterinary Medicine;2008-12
8 Ma dehui et al (Zhelimu Aninal Husbandry College Tongliao 028042);Study on Ultrastructure of Porcine Productive and Respiratory Syudrome (PRRS)[J];JOURNAL OF ZHELIMU ANIMAL HUSBANTRY COLLEGE;2000-01
9 RUAN Cheng-mai,CHEN Shou-yi,WU Dong-lin,LI Peng,MA He-wen,MA Yong-hong,ZHAN G Yu-jing (Faculty of Military Veterinary,Quartermaster University of PLA,Cha ngchun 130062,China);Expression of the Activity of Telomerase in Mammal Cells[J];Chinese Journal of Veterinary Science;2003-01
10 Hu Hui1,2, Gao Xiaoping2, Meng Hanbing1, Wang Yabin2, Chen Liying1, Zhang Hongying1, Xin Yinchuan2, Cui Bao'an2 (1College of Animal Science and Veterinary Medicine,Henan Argricultural University, Zhengzhou 450002; 2Henan Key Laboratory for Animal Food Safety, Zhengzhou 450002);Study on the proliferation profile of PRRSV in Marc-145 Cells Using Fluorescence Quantitative PCR[J];Chinese Agricultural Science Bulletin;2009-21
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Wang Ruimian, Yan Sixin Meng Yang ,Rong Chengxing(Department of Histology and Embryology , Department of Oral Anatomy and Physiology , Hubei Medical UniversityDepartment of Histology and Embryology.Hubei Medical University, Wuhan 430071 ,China;THE GROWTH AND PROLIFERATION OF HUMAN UMBILICAL ARTERY ENDOTHELIAL CELLS IN PRIMARY CULTURE[J];Acta Anatomica Sinica;1995-03
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