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《Chinese Journal of Animal and Veterinary Sciences》 2004-01
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Safety and Efficacy of PrV Ea TK~-/gE~-/gI~- Gene-deleted Vaccine

LIU Zheng-fei,CHENG Huan-chun,WU Bin,JIN Mei-ling,HE Qi-gai(College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 4370070,China)  
In this article,the safety and efficacy of PrV Ea TK~-/gE~-/gI~- gene deleted vaccine was studied systemically.The vaccine is safe when it was inoculated to pregnant sows,newborn piglets and finishing pigs with 10~(5.0)TCID_(50) and 10~(6.0)TCID_(50) vaccine dose and can protected sows after challenged with 10~(6.0)TCID_(50) PrV Ea field strain.GE antibody can not be produced in the serum of piglets a month later.The neutralization antibody increase greatly after second inoculation with the vaccine.Moreover PrV Ea TK~-/gE~-/gI~- vaccine strain is biologically very safe to rabbits,cat and milk goats.
【Fund】: 国家863计划项目(2002AA241341);; 国家自然科学基金资助项目(30170701)。
【CateGory Index】: S852.4
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【References】
Chinese Journal Full-text Database 4 Hits
1 ZHOU Guo-hui~(1,2),NI Jian-qiang~1,TIAN Zhi-jun~1,QIU Hua-ji~(1*),LI Hai-yan~1,TONG Guang-zhi~(1*) (1.National Key Laboratory of Veterinary Biotechnology,Harbin Veterianry Research Institute,Chinese Academy of Agricultural sciences,Harbin 150001,China;2.College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China);Construction of a Recombinant Pseudorabies Virus Expressing the HA Gene of H3N2 Subtype Swine Influenza Virus[J];Chinese Journal of Animal and Veterinary Sciences;2005-10
2 LU Qin-zhang,QIN Guang-shen,HUANG Wei-jian,XUN Yan-yun (College of Animal Science and Technique,Guangxi University,Nanning 530005,China);Amplification,Cloning and Sequence Analysis of gE Gene of Pseudorabies Virus B and W Strains[J];Chinese Journal of Animal and Veterinary Sciences;2005-10
3 LIU Zhi-jie,REN Hui-ying,WEN Jian-xin,LIU Wen-hua,ZOU Ling,HAN Xian-jie,WEI Xiao-xiao(College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China);Detection of Wild-type Porcine Pseudorabies Virus by Digoxigenin-labeled DNA Probe[J];Chinese Journal of Animal and Veterinary Sciences;2008-11
4 LAI Zhi1,ZHANG Wei-jian2,QI Shi-hua3,GAO Jun-feng1,MA Jing-jing1,WU Bi-qing1(1.Shanghai Chuanghong Biotech Co.,Ltd,Shanghai 201619,China;2.Shanghai Municipal Animal Innocuous Treatment Centre, Shanghai 201719,China;3.Wuhan Choppo Biological Corporation Co.,Ltd,Wuhan 430079,China);The Screening of Adjuvants for Swine Pseudorabies Live Vaccine(Bathar-K61 Strain)[J];Chinese Journal of Veterinary Drug;2013-07
【Citations】
Chinese Journal Full-text Database 4 Hits
1 CHEN Huan-chun, ZHOU Fu-chun, FANG Liu-rong, HE Qi-gai, WU Bin, HONG Wen-zhou (Lab of Animal Virology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China);Construction of TK~-/gG~-/LacZ~+ Mutant of Pseudorabies Virus Strain Ea[J];Chinese Journal of Virology;2001-01
2 GUO Wan-zhu, XU Zhi-wen, Wang Xiao-yu, CHENG Lu, WANG Yin, WANG Ming-shu (Sichuan Agricultural University, Yaan 625014, Sichuan, China);Construction of new generation Pseudorabies virus gene deleted vaccine and the study of biologic characteristics[J];JOURNAL OF SICHUAN AGRICULTURAL UNIVERSITY;2000-01
3 Liu Zhenfei Chen Huanchun He Qigai Zhou Fuchun Fang Liurong(Key Laboratory of Agricultural Microbiology,Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China);Construction of Pseudorabies Virus Ea TK~-/gE~-/gp63~- MutantStrain and the Study on Its Biological Property[J];Acta Microbiologica Sinica;2002-03
4 Chen Huanchun, Fang Liurong, He Qigai, Jin Meilin,Suo Xufeng, Wu Meizhou(Huazhong Agricultural University,Wuhan 430070);STUDY ON THE ISOLATION AND IDENTIFICATION OF THE EA STRAIN OF PSEUDORABIES VIRUS[J];CHINESE JOURNAL OF ANIMAL AND VETERINARY SCIENCES;1998-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HAN Jing-fang CHEN Rui-ai SHAO Ding-yong TANG Man-hua LIANG Gui-yi(Guangdong Dahuanong Animal Health Products Co.,Ltd.,Xinxing Guangdong 527400);Construction and Primary Application of Universal Transfer Vector Deleting TK of Pseudorabies Virus[J];;2009-15
2 CHEN Huan-chun, ZHOU Fu-chun, FANG Liu-rong, HE Qi-gai, WU Bin, HONG Wen-zhou (Lab of Animal Virology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China);Construction of TK~-/gG~-/LacZ~+ Mutant of Pseudorabies Virus Strain Ea[J];Chinese Journal of Virology;2001-01
3 LV Jian-qiang,CHEN Huan-chun,ZHAO Jun-long,FANG Liu-rong,HE Qi-gai,XIONG Fuoratory of Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China);The Development of Recombinant Pseudorabies Virus Expressing Porcine Parvovirus VP_2 Gene and the Study on Its Biological Characters[J];Chinese Journal of Virology;2004-02
4 FANG Liu-rong,XIAO Shao-bo,JIANG Yun-bo,NIU Chuan-shuang, ZHNAG Hui,CHEN Huan-chun (Laboratory of Animal Virology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China);Construction of the Recombinant Pseudorabies Virus TK~-/gG~-/GP5~+ Expressing GP5 of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and the Preliminary Study on Its Biological Characterization[J];Chinese Journal of Virology;2004-03
5 ZHAO Wu~(1,2),XIAO Shao-bo~1,FANG Liu-rong~1,JIANG Yun-bo~1,SONG Yun-feng~1,YAN Lin~1,YU Xiao-lan~1,CHEN Huan-chun~(1*)(1.Laboratory of Animal Virology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;2.Guangxi Veterinary Research Institute,Nanning 530001,China);Construction of the Recombinant Pseudorabies Virus TK~-/gE~-/VP22GP5~+ Expressing GP5 of Porcine Reproductive and Respiratory Syndrome Virus Fused with VP22 of Bovine Herpesvirus 1[J];Chinese Journal of Virology;2006-01
6 YANG Ming-fan,CUI Bao-an,ZHANG Su-mei,WANG Xue-bin,XU Duan-hong,YANG Jing-yuan,WANG Li(College of Animal Husbandry and Veterinary Medicine,Henan Agricultural University, Zhengzhou,Henan,450002,China);Immune Efficiency of inactivated Vaccine of Porcine Parvovirus and Pseudorabies Virus[J];Progress In Veterinary Medicine;2003-06
7 LIU Zhi-jie1,LI Ru-ju2,ZHOU Li1,ZENG Zhi-yong1,3,TANG De-yuan1,WANG Bin1,GAN Zhen-lei1,WANG Feng1(1.College of Animal Science,Guizhou University,Guiyang,Guizhou 550025;2.Guiyang Ruite Xinke Agro-Pastoral Co.Ltd,Guiyang,Guizhou 550025;3.Guizhou Laboratory of Animal Epidemic Diseases,Guiyang,Guizhou 550025,China);Identification of Pathogens Causing Porcine Reproductive Failure Diseases[J];Guizhou Agricultural Sciences;2011-08
8 DONG Hao1,WU Bo2,4,ZHANG Jian-ying3,HU Gui-xue4(1.College of Life Science,Jilin Agricultural University,Changchun 130118,China;2.Heilongjiang Veterinary Institute,Qiqihaer 161006,China;3.Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,Shenzhen 518067,China;4.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China);Establishment and Application of Transcription-Polymerase Chain Reaction for Identification of Field Isolate of Porcine Pseudorabies Virus and Vaccine Strain[J];China Animal Husbandry & Veterinary Medicine;2011-12
9 Huang Weijian; Wen Ronghui; Qin Aizhen; Yu Kelun; Liang Jiaquan; Liu Fang(College of Amimal Science and Technology, Guangxi Univ., Nanning ,530005);Isolation and Identification of the GB Strain of Pseudorabies Virus[J];;1998-S1
10 XIONG Yi 1,ZHU Wei 1,LIU Qi 2,GUO Jiangang 2,ZHAO Guoming 2,WEN Lixia 2(1. Gangxi University;2.Guangxi General Veterinary Agency);Isolation and identification of pig pseudorabies virus strain GXA in Guangxi[J];Guangxi Agricultural Science;2005-02
China Proceedings of conference Full-text Database 2 Hits
1 Cheng Guofu Tang Wanyong Hu Xueying Zhou Shiqi ( Huazhong Agricultural University, Wuhan,430070);Observation on histopathology of Piglets artificially Infected With EA Strain of Pseudorabies Virus[A];[C];2001
2 Chen Yang,Xu Zhi-wen~*,Gou Wan-zhu,Zhu Ling,Chen Di-shi,Wang Yin,Wang Xiao-yu (Animal Biological Technological Cnter of Sichuan Agricultural University,Ya'an 625014,China);Construction of the Pseudorabies Virus Expression Vector to Coexpressing Porcine Paravovirus VP2 Gene,Porcine Circovirus ORF2 Gene and a Marker Enhanced Green Fluorescence Protein Gene[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YUAN Zhang et al(Faculty of Animal and Technology,Sichuan Agricultural University,Yaan,Sichuan 625014);Research Progress in the Pseudorabies Virus-Vector[J];Journal of Anhui Agricultural Sciences;2007-06
2 Wang Qin; Guo Wanzhu; Lou Gaoming ;Yan qigui; Wang Mingshu; Yu Guanghai( Sichuan Agricultural University, Sichuan 625014 )Thymidine kinase (TK) deletion mutants (TK-) of Pseudorabies virus (PRV) Fa strain were constructed by molecular biological methods.;CONSTRUCTION OF THYMIDINE KINASE DELETION MUTANTS OF PSEUDORABIES VIRUS FA STRAIN[J];Chinese Journal of Virology;1996-04
3 LOU Gao ming (Key Laboratory of Veterinary Biotechnology of Guangdong Province,Guangzhou 510640,China);The Establishment and Application of PCR to Clinical Diagnosis of Pseudorabies[J];Chinese Journal of Virology;2002-02
4 ZHAO Jun-long,CHEN Huan-chun,LV Jian-qiang,XIAO Shao-bo,ZHOU Fu-chun (College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Hubei,WUHAN 430070,China);Study on Gene Immunity of Porcine Parvovirus Structural Protein Genes VP_1 and VP_2[J];Chinese Journal of Virology;2003-01
5 LV Jian-qiang,CHEN Huan-chun,ZHAO Jun-long,FANG Liu-rong,HE Qi-gai,XIONG Fuoratory of Ministry of Agriculture,Huazhong Agricultural University,Wuhan 430070,China);The Development of Recombinant Pseudorabies Virus Expressing Porcine Parvovirus VP_2 Gene and the Study on Its Biological Characters[J];Chinese Journal of Virology;2004-02
6 FANG Liu-rong,XIAO Shao-bo,JIANG Yun-bo,NIU Chuan-shuang, ZHNAG Hui,CHEN Huan-chun (Laboratory of Animal Virology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China);Construction of the Recombinant Pseudorabies Virus TK~-/gG~-/GP5~+ Expressing GP5 of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and the Preliminary Study on Its Biological Characterization[J];Chinese Journal of Virology;2004-03
7 YIN Wen-ling1,2,YIN Long-bo1,2,YE Wei-cheng2,SUN Xue-qiang3,YAO Huo-chun3,ZHANG Miao-tao1,WANG Yi-cheng2,ZHANG Cun2(1.College of Veterinary Medicine,Northwest Agriculture & Forestry University,Yangling 712100,China;2.Institute of Animal Husbandry and Veterinary Science,Zhejiang Academy of Agriculture Sciences,Hangzhou 310021,China;3.Veterinary Medicine College,Nanjing Agricultural University,Nanjing 210095,China);Construction of an Infectious Clone of Pseudorabies Virus Strain ZJ Genome Maintained as a Bacterial Artificial Chromosome[J];Chinese Journal of Virology;2010-04
8 WANG Wei~1 ZHANG Cun-gen~2 (~1Beijing Agricultural College, Beijing 102206; ~2Institute of Agricultural Economy in CAAS, Beijing 100081);Analyses on the Sustainable Development of Pig Industry in China[J];Journal of Beijing Agricultural College;2004-01
9 CAO Shan Jin, QIAN He Nian, FENG Jie, FU Tian Yun, YE Xue, YAO Yu (Gynecologic Oncology Center, Peking University People's Hospital, Beijing 100044);The construction and its co expression of bicistronic retroviral vector encoding human B7-1 and IFN-γ[J];Journal of Beijing Medical University;2000-06
10 HU Gejiletu,XU Rigan(Key Laboratory of Reproductive Biology of Mammals and Biotechnology,Academy of Life Science,Inner Mongolia University,Huhhot 010023,China);Analysis of Present Situation,Problems and Countermeasures of the Goat Husbandries in China[J];Grass-feeding livestock;2005-04
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 YUAN Zhang et al(Faculty of Animal and Technology,Sichuan Agricultural University,Yaan,Sichuan 625014);Research Progress in the Pseudorabies Virus-Vector[J];Journal of Anhui Agricultural Sciences;2007-06
2 Lü Jian-qiang1,HE Yun-wei2,LU Ti-kang1,HUA Qun-yi1,QIN Zhi-feng1,TAO Hong1,YANG Jun-xing1,RUAN Zhou-xi1(1.Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen,Guangdong,518010,China;2.Biocentury Transgene(China)Co.,Ltd,Shenzhen,Guangdong,518048,China);Development and Application of a Real-time Fluorescence PCR Assay for Pseudorabies Virus[J];Progress in Veterinary Medicine;2010-03
3 GUO Guang-jun1,2,3,LV Su-fang2,XIAO Yue-qiang2,BI Yan-li3,WEI Feng2,3,SHEN Zhi-qiang2,3,DING Zhuang1 (1.The College of Animal Husbandry and veterinary of Jilin University,Changchun,Jilin,130062,China; 2.Shandong Binzhou Animal Science & Veterinary Medicine Insitute,Binzhou,Shandong,256600,China; 3.Shandong Lvdu Bio-industry Company,LTD.,Binzhou,Shandong,256600,China);Progress on Detection Method of Pseudorabies Virus[J];Progress in Veterinary Medicine;2011-12
4 XU Yun-zhen1,2,LIN Rong-hua1,2,HE Wen-jin1,2,WU Zi-ming1,2,CHEN You-qiang1,2,CHEN Ru-kai2(1.College of Life Sciences,Fujian Normal University,Fuzhou 350108,China;2.Key Laboratory of Sugarcane Genetics and Breeding,Ministry of Agriculture,Fuzhou 350108,China);Preparation and Detection for Sugarcane SPSⅢ Intron Probes Labelled with Digoxgenin[J];Journal of Fujian Normal University(Natural Science Edition);2010-06
5 WANG Yang-jie1.2,LIU Fang1,HUA Jun2,MA Ling2,CHEN Zhong-wei2,ZHANG Wei3,HUANG Hong-mei2,CHEN Feng-lian2,WU Jian-min2(1.College of Animal Science and Technique,Guangxi University,Nanning 530005,China;2.Guangxi Veterinary Research Institute,Nanning 530001,China;3.Bobai Center for Animal Disease Control and Prevention,Bobai 537600,China);Isolation and Identification of GXBB Strain of Porcine Pseudorabies Virus and Sequence Analysis of gE Gene[J];China Animal Husbandry & Veterinary Medicine;2008-11
6 WANG Yang-jie1,2,SHI Sheng1,QIN Shao-min1,YUAN Shu-zhi3,XIE Bin3,SHAO Shuai3,LIU Zhi4,WU Jian-min2*(1 College of Animal Sciences and Technology,Guangxi University,Nanning 53005,China;2 Guangxi Veterinary Research Institute,Nanning 530001,China;3 Huipeng Animal Medicine Co.,Ltd,Nanning,Guangxi 530001,China;4 Rongxian Animal Health Inspection,Rongxian,Guangxi 537500,China);Epidemiological monitoring and emphasis on controlling porcine pseudorabies in Guangxi[J];Guangxi Agricultural Sciences;2009-03
7 WANG Chao;LIU Geng;ZHANG Lin-bo;College of Life Sicence,Jilin Agricultural University;College of Chinese Medicine Material,Jilin Agricultural University;;Detection of Residual DNA in Recombinant Interleukin-1 Receptor Antagonist(IL-1ra) Using Digoxigenin Labeled Probe[J];China Animal Husbandry & Veterinary Medicine;2013-11
8 MA Li;YANG Li-mei;XU Qian-qian;WANG Yan;ZHANG Ying;ZHANG Zhi-mei;GUO Shi-jin;SHEN Zhi-qiang;WANG Yan-ping;Shandong Binzhou Animal Science and Veterinary Medicine Academy;Shandong Lvdu Ante Veterinary Drug Industry Co.,Ltd.;;Application Progress of Procine Pseudorabies Virus gE Protein in the Wild-type Virus Diagnosis[J];China Animal Husbandry & Veterinary Medicine;2014-02
9 GUO Rui;TIAN Yong-xiang;Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Science;Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding;;Cloning of Hubei Strain gE Gene of Porcine Pseudorabies Virus and Sequence Analysis[J];Journal of Anhui Agricultural Sciences;2014-25
10 WU Bo,HE Shi-wei (Graduate School,Jilin Agricultural University,Changchun Jilin 130118);Isolation and Identification of Pseudorabies Virus[J];Jilin Animal Husbandry and Veterinary Medicine;2009-12
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Qin; Guo Wanzhu; Lou Gaoming ;Yan qigui; Wang Mingshu; Yu Guanghai( Sichuan Agricultural University, Sichuan 625014 )Thymidine kinase (TK) deletion mutants (TK-) of Pseudorabies virus (PRV) Fa strain were constructed by molecular biological methods.;CONSTRUCTION OF THYMIDINE KINASE DELETION MUTANTS OF PSEUDORABIES VIRUS FA STRAIN[J];Chinese Journal of Virology;1996-04
2 CHEN Huan-chun, ZHOU Fu-chun, FANG Liu-rong, HE Qi-gai, WU Bin, HONG Wen-zhou (Lab of Animal Virology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China);Construction of TK~-/gG~-/LacZ~+ Mutant of Pseudorabies Virus Strain Ea[J];Chinese Journal of Virology;2001-01
3 Li Sanxiug(Dept. of Animal Science and Veterinary Medicine, Hebei Agri. Univ.,Baoding, China);Research Advances in Pseudorabies[J];Journal of Agricultural University of Hebei;1989-01
4 Guo Wanzhu (Sichuan Agricultural University,Yaan,Sichuan,China);DETECTION OF PSEUDORABIES VIRUS BY ~(32)p LABELED DNA PROBE[J];Journal of Sichuan Agricultural University;1991-01
5 Wang Liu; Liu Xueli; Wang Mei; Tong Guongzhi; Lu Jingliang(State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Harbing);Molecular Cloning of Human Cytomegalovirus DNA and Preparation of Its Specific DNA Probes[J];BIOTECHNOLOGY;1994-04
6 WT5BZ]Zhang Yinmei,Guo Wanzhu,Wang Mingshu,Yan Qigui [WT5BX](Sichuan Agricultural University,Yaan 625014,China) [WT5HZ];CONSTRUCTION OF TRANSFER VECTOR PLASMID OF PSEUDORABIES VIRUS Fa STRAIN[J];Chinese Journal of Animal and Veterinary Sciences;2001-04
7 Yan Qigui,Guo Wanzhu,Yu Guangkai,Wang Qin,Lou Gaoming(Sichuan Agricutural University,Yaan);CONSTRUCTION OF PSEUDORABIES VIRUS Fa STRAIN WITH DELETIONS IN GLYCOPROTEIN gI AND gP63 GENES[J];ACTA VETERINARIA ET ZOOTECHNICA SINICA;1997-06
8 Chen Huanchun, Fang Liurong, He Qigai, Jin Meilin,Suo Xufeng, Wu Meizhou(Huazhong Agricultural University,Wuhan 430070);STUDY ON THE ISOLATION AND IDENTIFICATION OF THE EA STRAIN OF PSEUDORABIES VIRUS[J];CHINESE JOURNAL OF ANIMAL AND VETERINARY SCIENCES;1998-02
9 WangQin;GuoWanzhu;Yin Wenqi(Sichuan Agricultural University, Yaan,Sichuan Province 625014);Detection of Pseudorabies Virus by Biotinylated DNA Probe[J];VIROLOGICA SINICA;1996-03
10 ZHOU Fu chun, CHAN Huan chun , FANG Liu rong,WU Bin (College of Animal Science and Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China);Cloning and Identification of the TK of Gene Pseudorabies Virus Ea Strain[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1999-05
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