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《Chinese Journal of Animal and Veterinary Sciences》 2004-01
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Study on SDS-PAGE and Immunogenicity of Mycoplasma Hyopneumoniae Strain MY-99Membrane Protein

XIONG Yan~1,SUN Xia~2,GOU Lin~1(1.Sichuan Agricultural University,Yaan 625014;2.Profesional College ofFarming and Veterinary of Shandong,Weifang 261061,China)  
Membrane protein of Mycoplasma hyopneumoniae strain MY-99 was fractionally extracted by two methods of Triton X-100 and KI,in which 0.6 mol/L KI had a good soluble to protein.Molecular weights of the membrane protien were 14-100 KD,and molecular weights of the protective antigens were 36 KD and 64 KD by SDS-PAGE and Western-blotting.T lymphocyte transformation test justified that Mycoplasma hyopneumoniae membrane protein was not only nonspecific mitogen but also specific mitogen,in which,the rate of T lymphocyte transformation were 10.08% and 24.75% respectively(P0.05).Meanwhile,there was a high antibody titer in pigs blood immunized by the membrane protein,and the titer was 1∶2~(3.50) after 7 days,1∶2~(6.33) after 14 days and 1∶2~(7.83) after 21 days.
【Fund】: 四川省教育局重点科研项目资助(2001 2004)
【CateGory Index】: S852.4
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