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《Chinese Journal of Animal and Veterinary Sciences》 2004-01
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Development and Trait Identification of the Rapid Diagnosis Strips of the Infectious Bursal Disease

ZHANG Gai-ping,LI Xue-wu,YANG Yan-yan,DENG Rui-guang,LI Qing-mei(Institute of Biology and Technique Science,Henan Agricultural Sciences,Zhengzhong 450002,China)  
Based on the monoclonal antibodies (McAbs)with high specificity and affinity,the IBD strip was successfully prepared.The result revealed the IBD strip which did not cross react with NDV?ALV?EDS-76?IBV?ILTV?MDV was possessed of good specificity and good sensitivity.The IBD strip was 32-64 times as sensitive as that of the AGP and could recognize a variety of IBDV strains including attenuated and virulent virus.Compared with ELISA,the positive coincidence rate of the IBD strip was 100%.36 hours after infecting,the IBDV was tested in the bursal of the chicken.The diagnosis result was obtained in 1-2 minutes.The IBD strip is provided with specificity,sensitivity,celerity,and briefness and it does not require any professional skill or other reagent at all.The method realized "One-Step-Diagnosis" of chicken disease.
【Fund】: 国家"863"资助项目(2001AA249030)
【CateGory Index】: S854.4
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