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《Chinese Journal of Animal and Veterinary Sciences》 2004-01
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Cloning and Sequencing Analysis for Promoter-enhancer Region of pp38 Genes from Different Strains of Marek's Disease Virus

DING Jia-bo~1,ZHAO Wen-ming~2,JIANG Shi-jin~1,ZHANG Ji-yuan~1,WANG Zeng-fu~1,CUI Zhi-zhong~(1)(1.College of Animal Science,Shandong Agricultural University,Taian 271018,China;2.Science Reserch Department,Yangzhou University,Yangzhou 225009,China)  
The promoter-enhancer region of the pp38 phosphoprotein were amplified from genomic DNA of MDV RB1B,GD2,J-1-E,Mdll,Md5 and CVI988 strains by PCR technique.The amplified fragments were cloned into pGEM-T-easy vector,and the obtained positive clones were sequenced and compared with each other.This study shows that there are some absent mutants.The homology of these genes was more than 95.9%,and that most of the mutants are near the replication origin of MDV itself.
【Fund】: 国家自然科学基金(30070544)
【CateGory Index】: S852.65
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