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《Chinese Journal of Animal and Veterinary Sciences》 2004-01
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Study on Digoxigenin-Labeled Nucleic Acid Probe for Detection of Goose Parvovirus

BU Ri-e,WANG Jun-wei,WU Jin-hua,SUN Hong-mei,Ulrich.Neumann(Animal Medicine College,Northeast Agricultural University,Harbin 150030,China)  
The Goose parvovirus (GPV) 1880 bp NS1 DNA fragment was recuperated from its recombinant plasmid and labeled with Digoxigenin prepared DNA probe for detection of GPV.The hybridization assay result of specificity showed all the DNA of GPV strains were positive,but other nucleotide extracted from DPV,GPPV and allantoic fluid of the nuinoculated embryonating eggs were negertive.The hybridization assay result of sensitivity showed as little as 0.0224 ng of GPV DNA could be detected by DIG-labeled probe.This probe could also hybridizate with GPV neucleotide extracted from the clinical samples which were treated different ways.So this DIG-labeled DNA probe could be used to detect the GPV.
【Fund】: 黑龙江省"十五"攻关课题(GB01B50202)
【CateGory Index】: S852.65
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