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《Chinese Journal of Animal and Veterinary Sciences》 2004-01
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Experession of Nonstructural Protein Gene 3ABC of Foot-and-Mouth Disease Virus in E.coli

CAO Yi-mei,LIU Zai-xing,LU Zeng-jun,GUO Jian-hong,CANG Hui-yun,XIE Qing-ge(Lanzhou Veterinary Research Institute,CAAS,Lanzhou 730046,China)  
Foot-and-mouth disease virus(FMDV) nonstructural protein gene 3ABC was expressed in E.coli.59.1 Ku fusion protein could be tested by SDS-PAGE and can be recognized by the positive bovine serum of FMDV.Through gel thin layer scanning analysis,the amount of target protein is over 31.4% of the total bacteria protein.The expressed product was used as the antigen in an enzyme-linked immunosorbent assay,this assay allowed the differentiation of vaccinated from infected bovine.
【Fund】: 国家973课题(G19990119011);; 甘肃省科技攻关项目(GS993-A41-025);; 中国农业科学院院长基金(0028-16);; 社会公益基金;; "十五"国家科技攻关(2002BA514A-18-1)。
【CateGory Index】: S852.65
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【Co-references】
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1 Zhu Caizhu Lu Yonggan Qiu Xiaogao Zhao Qizu Xie Qingge Zhang Weide Yuan Meirong Yang Furong Zhang Qiang (Lanzhou Veterinary Research Institute CAAS,Lanzhou 730046);A POLYMERASE CHAIN REACTION FOR THE DETECTION OF FMDV IN TISSUES AND O-P SAMPLES[J];CHINESE JOURNAL OF VIROLOGY;1998-03
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