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《Chinese Journal of Animal and Veterinary Sciences》 2019-01
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Cloning,Tissue Expression and Inheritance Patterns of Porcine Placenta-specific 1(PLAC1) Gene

DENG Dadong;HONG Linjun;PAN Li;LIU Bang;YU Mei;Key Laboratory of Agricultural Animal Genetics,Breeding and Reproduction of Ministry of Education,College of Animal Science and Technology,Huazhong Agricultural University;College of Animal Science and Technology,South China Agricultural University;  
The objective of this study was to investigate the expression of porcine PLAC1 gene,and preliminarily identify its gene structure,biological function and inheritance patterns.The placenta tissue of pigs on 26,50 and 95 days of pregnancy were collected,the full-length of PLAC1 mRNA and its splicing variants were cloned by using the RACE-PCR(rapid-amplification of cDNA ends)and RT-qPCR(real-time quantitative PCR).Moreover,the expression patterns of PLAC1 gene in placenta of pigs was verified by in situ hybridization.In addition,the sequence variation of PLAC1 gene was detected.The results showed that there were 3 different transcripts of the PLAC1 gene in pigs,and the CDS regions of the 3 transcripts were identical,with a length of 525 bp,encoding 174 amino acids;The results of the expression profile and hybridization in situof PLAC1 gene indicated that the PLAC1 gene was specifically expressed in pig placentalchorionic epithelium,and the expression of PLAC1 mRNA was different during different stages of pregnancy.The expression of PLAC1 mRNA was significantly higher on 50 and 95 days of gestation in placenta of pigs than 26 days of gestation.The genotype of SNP on PLAC1 gene confirmed that it was a hemizygous gene,and followed the inheritance patterns of hemizygous gene.The experiment data provide a basis for exploring the biological function of porcine PLAC1 gene in placenta development.
【Fund】: 国家自然科学基金(31572370);; 湖北省自然科学基金创新群体项目(2018CFA015);; 中央高校基本科研业务费专项基金资助(2662018PY037)
【CateGory Index】: S828
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