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《Chinese Journal of Animal and Veterinary Sciences》 2019-01
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Effect of Duck LXRαGene Overexpression on Lipid Metabolism in Primary Hepatocytes and PC3 Cells

LUO Hualun;LI Wangui;ZHANG Yiyu;WU Lei;QIN Yuanyu;College of Animal Science,Guizhou University;Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region of Ministry of Education;The Grass Workstation of Liupanshui City;  
To explore the regulation mechanism of duck LXRαgene on fat deposition,in this experiment,the pEGFP-N3 was used as the vector to construct the LXRαgene eukaryotic overexpression vector carried HIS-tag that was transfected into the duck primary hepatocytes and PC3 cells,respectively.The over expression level of LXRαgene in duck hepatocytes and PC3 cells were detected by the duck HIS-tag kit,and the correlation between the over expression of LXRαgene in the two kinds of cells and the lipid index contents was analyzed,and the regulation mechanism of LXRαgene on lipid metabolism in duck hepatocytes and PC3 cells was explored.The results showed that the over expression of LXRαgene in both cells were significantly positively correlated with the contents of triglyceride(TG)and high-density lipoprotein(HDL)(P0.01),and significantly negatively correlated with the contents of total cholesterol(TC)and low density lipoprotein(LDL)(P0.01).The results reveal that over expression of LXRαgene has a positive regulatory effect on the anabolism of TG and HDL,and has a negative regulatory effect on TC and LDL,and the regulatory mechanisms in the two types of cells are similar.The results of this study will lay a theoretical foundation for the further study of the role of LXRαgene in the lipid metabolism network pathway and the solve of excessive fat deposition in duck body.
【Fund】: 国家自然科学基金(31160453)
【CateGory Index】: S834
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【Citations】
Chinese Journal Full-text Database 3 Hits
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【Co-citations】
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1 CHAI Chan-Juan;YANG Hui-Yu;YANG Zhi-Ming;LIANG Bin;YAN Feng;WU Rui;BIAN Yun-Fei;Department of Cardiology,the Second Hospital of Shanxi Medical University;;Effects of Ang( 1-7 ) and Ang Ⅱ on the Cholesterol Efflux in THP-1 Derived Foam Cells[J];中国动脉硬化杂志;2014-09
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