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《Chinese Journal of Animal and Veterinary Sciences》 2019-01
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The Effect of PNPLA5 Knockout on the Testis Morphology and Sperm Motility in Rats

HU Yanqing;XU Kui;WEI Yinghui;QIU Yiqing;ZHANG Xiuling;WANG Bingyuan;LIU Zhiguo;MU Yulian;LI Kui;Institute of Animal Science,Chinese Academy of Agricultural Sciences;  
The aim of this study was to investigate the effect of PNPLA5 gene on male rats' fertility.We used male PNPLA5 knockout rats(KO)as the experimental group and wild type rats(WT)as the control group,feeding them with regular diet.The epididymis spermatozoa and testis tissue were collected.We detected the pregnancy rate and average litter size by breeding assay,the sperm motility by Computer Aided Sperm Analysis(CASA),the expression level of mitochondria function related proteins(cytochrome C,Cytc and cytochrome C Oxidase subunit IV)in sperms were detected by Western blot,and histochemical morphology of testis tissue by HE staining.The results showed that PNPLA5 knockout significantly reduced the pregnancy rate of the mated female rats(P0.01),significantly decreased the curvilinear velocity of sperm(P0.05)and percentage of sperm with progressive movement(P0.01)compared to the wild type male rats.We also found that the PNPLA5 knockout downregulated the expression level of Cytc and upregulated expression level of COX IV in sperms compared with those in wild type rats(WT),but no significant difference were observed between 2groups(P0.05).In addition,the histological structure of testis of PNPLA5 knockout rats(KO)appeared loose,unarranged,and epithelial cells detached to the lumen.In conclusion,this study shows that PNPLA5 knockout reduces the fertility of male rats and it may provide an important evidence for understanding the animal reproductive performance.
【Fund】: 国家自然科学基金(31572378);; 中央级公益性科研院所基本科研业务费专项资金项目(2016ywf-yb-4);; 中国农业科学院科技创新工程(ASTIP-IAS05)
【CateGory Index】: S814
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