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《Chinese Journal of Animal and Veterinary Sciences》 2019-01
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Cloning and Expression of Lin28A Gene in the Onset of Puberty in Duolang Sheep

XING Feng;GAO Qinghua;QI Xin;LI Qingjin;LI Chuang;College of Animal Science,Tarim University;Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production & Construction Corps;  
The objectives of the present study were to clone the Lin28AcDNA of sheep,investigate its expression patterns and regulation properties during the onset of puberty in sheep.In this study,5Duolang sheep were slaughtered in the prepuberty(1week before the first estrus),puberty and postpuberty(1week after the first estrus),respectively,hypothalamus,pituitary and ovarian tissues were collected.The Lin28A cDNA of these tissues was cloned,the homology of the amino acids of Lin28Abetween Duolang sheep and other species were compared by DNAman6.0.The expression patterns of Lin28A,let-7aand let-7bin hypothalamus,pituitary and ovary during the puberal transition in Duolang sheep were detected by real-time PCR.The results showed that the fragment of Lin28AcDNA was 3 581bp which contained 2 963bp of 3′UTR and 618 bp of the coding region.The deduced protein sequence of the Lin28A coding region shared87.56%,88.52%,92.68%,89.95%,76.10% and 61.84% to that of human,house mouse,cattle,sheep,original chicken and zebrafish.The expression of Lin28A was significantly decreased in the hypothalamus and ovary from prepuberty to puberty (P0.05),the expression levels of let-7aand let-7b did not change significantly(P0.05).The results provided a foundation for revealing the function of Lin28A gene and its role in the onset of puberty in sheep.
【Fund】: 国家自然科学基金(31660652);; 兵团科技计划项目(2015AG013);; 塔里木畜牧科技重点实验室开放课题(201504)
【CateGory Index】: S826
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