Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Animal and Veterinary Sciences》 2019-01
Add to Favorite Get Latest Update

Cloning and Expression of Lin28A Gene in the Onset of Puberty in Duolang Sheep

XING Feng;GAO Qinghua;QI Xin;LI Qingjin;LI Chuang;College of Animal Science,Tarim University;Key Laboratory of Tarim Animal Husbandry Science and Technology,Xinjiang Production & Construction Corps;  
The objectives of the present study were to clone the Lin28AcDNA of sheep,investigate its expression patterns and regulation properties during the onset of puberty in sheep.In this study,5Duolang sheep were slaughtered in the prepuberty(1week before the first estrus),puberty and postpuberty(1week after the first estrus),respectively,hypothalamus,pituitary and ovarian tissues were collected.The Lin28A cDNA of these tissues was cloned,the homology of the amino acids of Lin28Abetween Duolang sheep and other species were compared by DNAman6.0.The expression patterns of Lin28A,let-7aand let-7bin hypothalamus,pituitary and ovary during the puberal transition in Duolang sheep were detected by real-time PCR.The results showed that the fragment of Lin28AcDNA was 3 581bp which contained 2 963bp of 3′UTR and 618 bp of the coding region.The deduced protein sequence of the Lin28A coding region shared87.56%,88.52%,92.68%,89.95%,76.10% and 61.84% to that of human,house mouse,cattle,sheep,original chicken and zebrafish.The expression of Lin28A was significantly decreased in the hypothalamus and ovary from prepuberty to puberty (P0.05),the expression levels of let-7aand let-7b did not change significantly(P0.05).The results provided a foundation for revealing the function of Lin28A gene and its role in the onset of puberty in sheep.
【Fund】: 国家自然科学基金(31660652);; 兵团科技计划项目(2015AG013);; 塔里木畜牧科技重点实验室开放课题(201504)
【CateGory Index】: S826
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LU Ping-ping;XING Feng;LIU Bo-dan;LIANG Zhi-peng;College of Animal Science,Tarim University;Key laboratory lof Tarim Animal Husbandry Science and Technology,XinJiang Production & Construction Corps;;Lin28B Gene Expression and its Role in the Onset of Puberty of Sheep[J];家畜生态学报;2014-09
2 LIU Xue-rong~(1,2,#),TIAN Wen-hong~(2,3#),DONG Xiao-yan~4,WU Xiao-zhe~(2,5),LV Jian-xin~(1*),WU Xiao-bing~(2*) (1.Zhejiang Provincial Key Laboratory of Medical Genetics,Wenzhou Medical College,Wenzhou 325035,China;2.State Key Laboratory for Molecular Virology and Genetic Engineering,Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China;3.School of Life Science,]ilin University,Changchun 130012,China;4.Beijing Fiveplus Molecular Medicine Institute,Beijing 100176,China;5.State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu 610041,China);Effects of Lin28a and Lin28b on let-7 Family Activity[J];病毒学报;2011-06
3 CHEN Chao,CHEN Ju-xiang Department of Neurosurgery,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;Function of Lin28:an advance[J];第二军医大学学报;2011-02
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Han Ziwu;Zhou Mingjie;Fan Chunlei;College of Life Science, Zhejiang Traditional Chinese Medical University;;Research of the Relationship between Lin28 and the Paclitaxel-resistant of Hepatocellular Carcinoma Cell Lines[J];浙江中医药大学学报;2014-07
2 PEI Li-guo;QU Lei;JIA Fei;ZHANG Chuan;GUO Meng-jing;LU Hong;HUO Zheng-hao;Ningxia Medical University,Key Laboratory of Fertility Preservation and Maintenance,Ministry of Education,Key Laboratory of Heredity and Reproduction of Ningxia;Department of Basic Medicine of Ningxia Medical University;;LIN28B gene polymorphisms in Ningxia Hui and Han ethnic people[J];解剖学报;2014-01
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 HAN Yue - zhen~(1,2),HUANG Xi - xia~1,DI Jiang~2,TIAN Ke - chuan~2,WU Wei - wei~2,XU Xin - ming~2, Hanikezi~2,FU Xue - feng~2,ZHANG Yan - hua~2,Mayila~1,Aimaiti~1 (1.College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China;2.Xinjiang Academy of Animal Sciences,Urumqi 830000,China);Development of Real - time PCR for GAPDH Gene in the Skin Tissues of Merino Sheep[J];新疆农业科学;2012-10
2 HAN Wei1,2,LI Hui-fang1,ZHU Yun-fen1,SONG Chi1,TAO Zhi-yun1(1.Poultry Institute,Chinese Academy of Agricultural Science,Yangzhou,Jiangsu 225125,China; 2.Nanjing Agricultural University,Nanjing,Jiangsu 210095,China);Regulation Mechanism of Puberty Onset in Animals[J];家畜生态学报;2012-05
3 WU Xiaobing, DONG Xiaoyan, WU Zhijian, CAO Hui, NIU Dongbin, QU Jianguo, WANG Hong & HOU YundeState Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100052, China;Laboratory of Morphology, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China;A novel method for purifica- tion of recombinant adeno-associated virus vectors on a large scale[J];Chinese Science Bulletin;2001-06
4 WU Zhijian, WU Xiaobing and HOU Yunde State Key Laboratory for Molecular Virology and Genetic Engineering , Institute of Virology , Chinese Academy of Preventive Medicine , Beijing 100052, China Corresponding author;Generation of a recombinant herpes simplex virus which can provide packaging function for recombinant adeno-associated[J];Chinese Science Bulletin;1999-08
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved