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《Chinese Journal of Animal and Veterinary Sciences》 2019-01
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Integrated Analysis of miRNA and mRNA Network of Porcine Circovirus 2 Infecting Porcine Kidney Cell Line

LI Chang;LI Jing;HE Qigai;State Key Laboratory of Agricultural Microbiology,Huazhang Agricultural University;College of Animal Science and Veterinary Medicine,Huazhong Agricultural University;  
The purpose of this study was to elucidate changes in miRNA and mRNA expression profiles of PCV2 infected PK-15 cells,and to illuminate the interaction between differentially expressed miRNAs and mRNAs.Stem-loop quantitative PCR was used to detect and identify differential expression levels of multiple hot spot miRNAs in PCV2 infected and uninfected groups.The results of proteomic study of PCV2 infected cells at the 12 th hour post infection(hpi)were analyzed(GEO Accession No.:GSE71945)to obtain differentially expressed mRNA and to analyze the interaction between differentially expressed miRNA and mRNA.It was found that miR-10 b,miR-128,miR-155-5p,miR-21,miR-29 b,miR-361-3p were differentially expressed at 12 hpi.By using miRecords to predict their target genes,7 105,9 741,7 451,7 183,7 971and8 793 target genes were found,respectively.Totally there were 158 differentially expressed mRNAs that belonged to various cell components and participated in many important biological pathways,and there was a large number of common target genes between the differentiallyexpressed miRNAs candidate target genes and differentially expressed mRNAs.In summary,PCV2 infection can lead to disruption of multiple miRNA expression in cells,resulting in disorganization of miRNA target genes,which in turn influences the normal life events of cells.
【Fund】: 国家生猪产业技术体系资助项目(CARS-35)
【CateGory Index】: S858.28
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