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《Chinese Journal of Animal and Veterinary Sciences》 2019-01
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Isolation and Identification of Streptococcus suis Causing Meningitis in Piglets

CHEN Shenshen;MA Jiale;ZHANG Xinfeng;QU Xiangyang;PAN Zihao;YAO Huochun;College of Veterinary Medicine,Nanjing Agricultural University;OIE Reference Laboratory for Swine Streptococcus of Nanjing Agricultural University;The Hog-rasing Department of Huanshan Group;  
The purpose of this study was to isolate and identify pathogenic bacteria from brain tissues and other organs of piglets suffering from meningitis.The species and serotype of the isolated strain were identified by PCR and serum agglutination test.The ultrastructure of the isolated strain was observed by transmission electron microscope,and the sequence type(ST)was determined by multilocus sequence typing tests(MLST).Minimal inhibitory concentration(MIC)was determined to analyze the antibiotic resistance of the isolated strain,while the virulence was detected by mice infection experiments.Results were as follows:a strain of Streptococcus suis was isolated from the brain tissue of piglets with meningitis which belongs to serotype 9and named WH1609.The result of electron microscope showed that WH1609 had capsular structure.The result of MLST showed that WH1609 belonged to a new ST.WH1609 was a multi-drug resistant strain but sensitive to vancomycin,penicillin and ampicillin.The LD_(50) of BALB/c mice infected with WH1609 was 1.89×10~2 CFU·mL~(-1),and histopathological observation showed that the in-fected mice had obvious lesion in brain tissue.All the results suggest that a virulent strain of Streptococcus suis 9isolated from the brain tissue of piglets suffering from meningitis exhibited a 10~5 times higher LD_(50) than that of other Streptococcus suis virulent strains,which is of great value to the study of the molecular mechanism meningitis caused by Streptococcus suis.
【Fund】: 上海市科技兴农重点攻关项目[沪农科攻字(2016)第6-2-1号]
【CateGory Index】: S852.61
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Chinese Journal Full-text Database 3 Hits
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【Co-citations】
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1 HAN Shao-peng;YU Xiang-qian;YE Cheng-rong;ZHU Jian-guo;Shanghai Key Laboratory of Veterinary Biotechnology,School of Agriculture and Biology,Shanghai Jiaotong University;Pudong New District Animal Disease Prevention and Control Center;;Identification and Drug Resistance Analysis of Streptococcus suis Type 2 from the Pudong Area[J];上海交通大学学报(农业科学版);2015-04
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