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《Chinese Journal of Animal and Veterinary Sciences》 2019-01
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Prokaryotic Expression and Primary Evaluation of Antibacterial Activity of Antibacterial Factor in Baylisascaris schroederi

WU Yiran;GU Xiaobin;XIE Yue;YANG Guangyou;College of Veterinary Medicine,Sichuan Agricultural University;  
This experiment was conducted to explore the characterization and the antibacterial activity of an antibacterial factor in Baylisascaris schroederi.The gene BsABF was cloned and expressed with prokaryotic expression system.Western blot and immunofluorescence histochemical staining were used to determine the immunological characteristics and the location in the parasite.The antibacterial activity was evaluated by the preliminary antibacterial experiments.Results were as follows:Open reading frame of BsABFgene (276bp) encoded a 10.01 kDa protein with an N-terminal signal peptide while sharing 94% identity with Ascaris suum.The protein is mainly distributed in hypodermis,intestinal and eggs.The prokaryotic expression of rBsABF had a good immunoreactivity.Meanwhile the antibacterial assessment revealed that the recombinant protein has the antibacterial activity towards Enterococcus faecalis and Escherichia coli in giant pandas.In this study,we have first identified the BsABFgene,and rBsABF does have the antibacterial activity,and it lays the foundation for further study on the functional properties of BsABF in the future.
【Fund】: 成都大熊猫繁育研究基金项目(CPF2017-24)
【CateGory Index】: S852.7
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