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《Chinese Journal of Animal and Veterinary Sciences》 2019-01
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The Effect of 17β-estradiol on the Ca~(2+) Concentration in DRG Neurons of Newborn Rabbits

GUO Yaru;ZHANG Jin;ZHANG Yingli;ZHAO Jianshuai;DU Yi'nan;XIN Xianmeng;XU Yongping;College of Veterinary Medicine,Northwest A&F University;  
The study was conducted to investigate whether estrogen can affect the regulation of primary sensory neurons,and to explore the role of Ca~(2+) in this mechanism.The distribution of estrogen receptor(ER)in primary culture dorsal root ganglion (DRG) from newborn rabbits was observed by Immunocytofluorescent(ICF),and the effects of 17β-E_2(1,10,100,1 000nmol·L~(-1))on cytosolic free Ca~(2+) fluorescence in DRG neurons cultured for 72 hwere monitored by Laser Scanning Confocal Microscopy(LSCM).It was found that,in primary cultured DRG neurons,ERα,ERβand GPR30 were 45.61%,32.39%,39.82% positive or strongly positive neurons respectively,and the neurons proportions of different sizes in different ER positive or strongly positive neurons were different.ERαand ERβwere strongly positive in DRG neuron nuclei,me-dium positive in cytoplasm,and weakly positive in a little neurite.GPR30 were weakly positive or negative in DRG neuron nuclei,medium positive or strongly positive in cytoplasm,and weakly positive in neurite.A certain degree of "heterogeneity" of three types of ERs(ERα,ERβ,and GPR30)was observed in the number,size type,degree of response and distribution in positive neurons.According to the variation of Ca~(2+) fluorescence intensity induced by 17β-E_2,DRG neurons were divided into 3types,excitatory(19.28±0.70)%,inhibitory(3.54±0.02)% and insensitive neurons(77.17±1.48)%,respectively.Excitatory neurons were divided into strongly excitatory neurons(0.195±0.118)and weakly excitatory neurons(0.032±0.003)depending on the intensity of Ca~(2+) fluorescence changes.Interestingly,with the increase of 17β-E_2,the proportion of strongly excitatory neurons decreased,but no difference was detected in the proportion of total excitatory neurons at each group.It was indicated that with the increasing of 17β-E_2,the increasement of[Ca~(2+)]_iwas inhibited in strongly excitatory neurons,but not completely inhibited.These results suggest that estrogen may have distinct effects on various sensory neurons;Ca~(2+) pathway is one of the mechanisms in the effects of estrogen on the activity of some primary sensory neurons,and estrogen inhibits the effect of Ca~(2+) in DRG neurons in two ways:a direct inhibition of the effect of Ca~(2+) signaling pathway in several neurons,and a certain inhibitory impact on the effect of Ca~(2+) increment in strongly excitatory neurons with the increasing estrogen.
【Fund】: 陕西省农业推广项目(K332021401)
【CateGory Index】: S829.1
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