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《Chinese Journal of Animal and Veterinary Sciences》 2019-01
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The Effect of 17β-estradiol on the Ca~(2+) Concentration in DRG Neurons of Newborn Rabbits

GUO Yaru;ZHANG Jin;ZHANG Yingli;ZHAO Jianshuai;DU Yi'nan;XIN Xianmeng;XU Yongping;College of Veterinary Medicine,Northwest A&F University;  
The study was conducted to investigate whether estrogen can affect the regulation of primary sensory neurons,and to explore the role of Ca~(2+) in this mechanism.The distribution of estrogen receptor(ER)in primary culture dorsal root ganglion (DRG) from newborn rabbits was observed by Immunocytofluorescent(ICF),and the effects of 17β-E_2(1,10,100,1 000nmol·L~(-1))on cytosolic free Ca~(2+) fluorescence in DRG neurons cultured for 72 hwere monitored by Laser Scanning Confocal Microscopy(LSCM).It was found that,in primary cultured DRG neurons,ERα,ERβand GPR30 were 45.61%,32.39%,39.82% positive or strongly positive neurons respectively,and the neurons proportions of different sizes in different ER positive or strongly positive neurons were different.ERαand ERβwere strongly positive in DRG neuron nuclei,me-dium positive in cytoplasm,and weakly positive in a little neurite.GPR30 were weakly positive or negative in DRG neuron nuclei,medium positive or strongly positive in cytoplasm,and weakly positive in neurite.A certain degree of "heterogeneity" of three types of ERs(ERα,ERβ,and GPR30)was observed in the number,size type,degree of response and distribution in positive neurons.According to the variation of Ca~(2+) fluorescence intensity induced by 17β-E_2,DRG neurons were divided into 3types,excitatory(19.28±0.70)%,inhibitory(3.54±0.02)% and insensitive neurons(77.17±1.48)%,respectively.Excitatory neurons were divided into strongly excitatory neurons(0.195±0.118)and weakly excitatory neurons(0.032±0.003)depending on the intensity of Ca~(2+) fluorescence changes.Interestingly,with the increase of 17β-E_2,the proportion of strongly excitatory neurons decreased,but no difference was detected in the proportion of total excitatory neurons at each group.It was indicated that with the increasing of 17β-E_2,the increasement of[Ca~(2+)]_iwas inhibited in strongly excitatory neurons,but not completely inhibited.These results suggest that estrogen may have distinct effects on various sensory neurons;Ca~(2+) pathway is one of the mechanisms in the effects of estrogen on the activity of some primary sensory neurons,and estrogen inhibits the effect of Ca~(2+) in DRG neurons in two ways:a direct inhibition of the effect of Ca~(2+) signaling pathway in several neurons,and a certain inhibitory impact on the effect of Ca~(2+) increment in strongly excitatory neurons with the increasing estrogen.
【Fund】: 陕西省农业推广项目(K332021401)
【CateGory Index】: S829.1
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【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 WU Wei-ni,XU Yong-ping,WANG Hong-mei,FAN Jie,WANG Zhi-hao,MING Jia(College of Veterinary Medicine,Northwest Sci-Tech University of Agriculture and Forestry,Yangling 712100,China);The expression of ER-α and ER-β in inferior mesenteric ganglion of female goat[J];中国兽医杂志;2012-04
2 ZHAO Hui-ying,XU Li,JIANG Zhen-xing,XIONG Dong-sheng,GENG Yang-xue (College of veterinary medicine,Northwest A&F University,Yangling,Shaanxi 712100,China);Expression of ERα in the stomach and small intestine of rats under different nutritional conditions[J];中国兽医杂志;2011-11
3 LIU Xiao-Liu,LI Guang-Qian.Department of Anatomy,Medical College of Whuan University of Science and Technology,Wuhan 430081,China;The influence of estrogen on expression of interleukin-6 and nerve growth factor in cardiac ganglia in female rats[J];中国免疫学杂志;2009-08
4 FAN Guan-wei, HE Jun, WANG Hong, GAO Xiu-meiResearch Centre of Traditional Chinese Medicine, Tianjin University of TCM, Tianjin 300193, China;Advances in study of estrogen and its receptors signal transduction pathway[J];中国临床药理学与治疗学;2007-03
5 Chen Xiaowu, Zhu Shanle, Fan Yuhua, Zhao Xiaoming(Department of anatomy of Xianning Medical College, Xianning 437100; Huazhong University of Science and Technology, Tongji Medical College, Wuhan 430030);THE EFFECTS OF ESTROGEN ON THE EXPRESSION OF Bcl-2 AND Bax IN THE INTRACARDIC GANGLIONIC NEURONS OF THE OVARIECTOMIZED FEMALE RATS[J];神经解剖学杂志;2004-05
6 CHEN Xiao\|wu, ZHU Shan\|le * , YIN Shu\|yi, FAN Yu\|hua, ZHAO Xiao ming (Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan\ 430030,China) \;[;EXPRESSION OF ESTROGEN RECEPTOR PROTEIN AND mRNA IN INTRACARDIAC GANGLIA IN FEMALE RATS[J];解剖学报;2003-04
7 Han wei, Li Xuehui, Li Yanqing, et al. Department of Gastroenterology,Qilu Hospital,Shandong University,Jinan 250012;The functional research of expression of estrogen receptors and progestogen receptors of intestinal mucosa in the irritable bowel syndrome pathogenesis[J];胃肠病学和肝病学杂志;2002-04
8 CHANG Qing, YING Da jun, SHI Chang xu (Department of Obstetrics and Gynecology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effects of estrogen on neuron structure and expression of estrogen receptor in hypotha- lamus[J];第三军医大学学报;2001-03
9 Tong Xiaojie, Yu Pin,Yoshiaki Nojyo 1 (Department of Anatomy, College of Basic Medical Sciences, China Medical University, Shenyang,110001);Origin, Run and Distribution of Preganglionic Nerve Fibers in Celiac-Superior Mesenteric Ganglion of the Rat[J];中国医科大学学报;2000-02
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