Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Animal and Veterinary Sciences》 2019-01
Add to Favorite Get Latest Update

Establishment of Vero/SLAM Cell Lines with Stable Expression SLAM to Enhanced PPRV Replication Using the Lentiviral Expression System

WU Jinyan;TIAN Hong;MENG Xuelian;HUI Xiaoting;WANG Yaojie;GUAN Yuhua;SHANG Youjun;LIU Yongsheng;ZHANG Zhidong;Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;  
By the Gateway technology and the lentiviral expression system,goat signaling lymphocyte activation molecule(SLAM,also known as CD150)was stably integrated into the genome chromosome of Vero cell,to establish a Vero/g-SLAM positive cell clones,with which the ability of replication of peste des petits ruminants virus(PPRV)can be improved.On the other hand,to verify the activity,genetic stability and proliferation of PPRV expressing SLAM of Vero/g-SLAM.Peripheral blood lymphocytes were isolated from goat,of which genomic total RNA was extracted,SLAM gene with complete ORF were obtained by one step RT-PCR.Using of the BP and LR loci gene recombination technology to construct the entry clone vector pDONR/SLAM and expression skeleton of pDEST/SLAM,with packaging plasmids pLP1,pLP2 and VSV-G to co-transfect 293-FT cells to produce a lentiviral stock,use the lentiviral stock to infect Vero cell.The Vero cells with SLAM were named Vero/SLAM,and were resistant to the blasticidin(3.5μg·mL~(-1)).By the target gene amplification,Confocal laser scanning microscope,indirect immunofluorescence,Western blot and cytopathic effect,the SLAM genome integration,transcription,expression and reaction activity of SLAM protein,and PPRV proliferation effect in Vero/SLAM were verified respectively.Results were as follows:SLAM was stably integrated in the genome of Vero cells;The receptor protein was expressed in the periplasmic part of the cell;After continuous subpassages of the cells,SLAM are not lost;Vero/SLAM were inoculated virus The cytopathic time was shortened from 4-7dto 3.5d,and TCID_(50)·0.1mL~(-1) increased from4.25 to 5.67.Compared with the normal Vero cells,Vero cells that integrate SLAMhave significantly enhanced their susceptibility to PPRV because of expression of PPRV specific cell receptors.In this study,a new cell line,Vero/SLAM cells was established successfully.The SLAM expressed by this cell has the obvious function of enhancing PPRV replication,the critical target genes that enhance PPRV replication can be elucidated at the cellular model level.It also provides information for the study of host cell changes caused by PPRV infection and the pathogenesis of the organism.
【Fund】: 国家现代肉羊产业技术体系项目(CARS-39-04B);; 甘肃省科技支撑计划项目(1604NKCA059);; 内蒙科技重大专项“巴美肉羊产业化技术研究与集成应用”
【CateGory Index】: S852.65
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 7 Hits
1 ZHANG Jia-lin;LI Cui-cui;XIE Mei-mei;WANG Zi-long;CHEN Wei-ye;BU Zhi-gao;State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences;;The establishment and application of BHK-21 expressing goat SLAM[J];中国预防兽医学报;2015-07
2 ZHU Ying;LIU Wen-xin;ZHAO Shu-jing;LI Dan-dan;FENG Yu-fei;GUAN Wei-kun;HE Li-li;JIANG Kui-yu;SHI Dong-fang;College of Veterinary Medicine, Northeast Agricultural University;Center of Major Animal Disease Emergency in Liaoning Province;;Establishment of BHK-21 cell line stably expressing caine distemper virus recepter of signaling lymphocyte activation molecule[J];中国预防兽医学报;2014-06
3 ZHAO Rong;RONG Qi-xian;LIU Yu-zhong;SHEN Ye;HUANG Lu-qi;Heilongjiang University of Chinese Medicine;Natinal Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Scinences;;Construction of RNAi vectors for SmNAC1 transcription factors of Salvia miltiorrhiza using Gateway cloning technology[J];中国中药杂志;2014-09
4 ZHOU Jie;WANG Xyu-ming;Chen Bin;CHEN Juan;YANG Yong;YYU Chu-lang;YAN Cheng-qi;CHEN Jian-ping;State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control,MOA Key Laboratory for Plant Protection and Biotechnology,Zhejiang Provincial Key Laboratory of Plant Virology,Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences;College of Chemistry and Life Sciences,Zhejiang Normal University;;Low-cost gateway-compatible bimolecular fluorescence complementation assay system[J];浙江农业学报;2013-05
5 Jianjun Zhao1,Ruxun Yan2,Hailing Zhang1,Lei Zhang1,Bo Hu1,Xue Bai1,Xiqun Shao1,Xiuli Chai1,Xijun Yan1,2,Wei Wu1,2 1 Division of Preventive Veterinary Medicine,Institute of Special Economic Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Changchun 130112,China 2 Jilin Teyan Biotechnological Co.Ltd,Changchun 130122,China;Establishment and application of a Vero cell line stably expressing raccoon dog SLAM,the cellular receptor of canine distemper virus[J];微生物学报;2012-12
6 WANG Le-yuan1,2,Tse Drum3,WU Guo-zhen4,AN Rong-xiang5, ZHOU Ming6,Duojie Redeng7,CAI Bin3 (1.Veterinary Service Bureau of Tibet Autonomous Region,Lhasa 850000,China;2.China Institute of Veterinary Drug Control,Beijing 100081,China;3.Department of Agriculture and Animal Husbandry,Tibet Autonomous Region,Lhasa 850000,China;4.Center for Animal Disease Control,Tibet Autonomous Region,Lhasa 850000,China;5.Institute of Animal Health and Quarantine,Tibet Autonomous Region,Lhasa 850000,China;6.Ngari Agriculture and Animal Husbandry Bureau,Ngari 859000,China;7.Nakchu Agriculture and Animal Husbandry Bureau,Nakchu 852000,China);Occurrence and Control Strategy of Peste Des Petits Ruminants in Tibet Autonomous Region of China[J];畜牧兽医学报;2011-05
7 ;Diagnosis of the First Outbreak of Peste des Petits Ruminants in Tibet[J];中国动物检疫;2007-08
Chinese Journal Full-text Database 10 Hits
1 FENG Jia-wang;LI Feng-sai;GU Qing-yun;WEN Min;YANG Kang-lin;DAWA Zhuo-ma;HOU Shao-hua;JIN Hong-yan;Tibet Vocational Technical College;College of Veterinary Medicine,Northeast Agricultural University;Institute of Animal Husbandry and Veterinary Medicine,Chinese Academy of Agricultural Sciences;;Progress on Measles Virus Reverse Genetics[J];动物医学进展;2015-12
2 CAO Dong-shen;ZHAO Bo-lin;QU Ping;HU Dong-mei;WANG Wen-chao;SONG Xiao-hui;PAN Shu-de;Shenyang Agricultural Uinversity;China Animal Disease Prevention and Control Center;;Progress on Genetic Engineering Vaccines of Peste Des Petits Ruminants[J];动物医学进展;2015-11
3 LI Ru;ZHOU Jie;LI Dong-yue;WANG Xu-ming;YANG Yong;YU Chu-lang;CHENG Ye;YAN Cheng-qi;CHEN Jian-ping;College of Plant Protection,Nanjing Agricultural University;State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control,Key Laboratory of Biotechnology in Plant Protection,Ministry of Agriculture/Zhejiang Provincial Key Laboratory of Plant Virology/Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences;College of Chemistry and Life Sciences,Zhejiang Normal University;;Expression of OsWRKY7 in Rice[J];中国水稻科学;2015-06
4 Ge Shengqiang;Wu Xiaodong;Zhang Zhicheng;Zhang Yongqiang;Zhao Yonggang;Liu Hualei;Wang Zhiliang;China Animal Health and Epidemiology Center;;A Comparison of the Emergency Plans for Peste des Petits Ruminants between Australia and China[J];中国动物检疫;2015-07
5 WANG Guang-xiang;JIA Ning;FANG Mei;SUN Bao;College of Veterinary Medicine,Gansu Agricultural University;Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences;;Studies on the Pathology of the Peste des Petits Ruminants of Sheep[J];畜牧兽医学报;2015-06
6 SU Feng-yan;LI Zhe;LIU Qian-hui;WANG Quan-kai;College of Chinese Medicinal Materials,Jilin Agricultural University;;Research Progress on Diagnostic Technique of Canine Distemper[J];中国畜牧兽医;2015-05
7 MA Ying;MA Xiao-hui;MA Xiao-jing;GUO Juan;HUANG Lu-qi;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;Pharmacy College,Chengdu University of Traditional Chinese Medicine;;RNA interference and its effect of CYP76AH1 in biosynthesis of tanshinone[J];中国中药杂志;2015-08
8 LIU Yong-hong;ZHAO Li;CAO Sheng-bo;LI Long-kai;WANG Hai-guo;LIAO Qiu-ping;JIAO Hai-hong;College of Animal Science,Tarim University,Key Laboratory of Tarim Animanl Husbandry Science and Technology of Xinjiang Production & Construction Corps;College of Veterinary Medicine,Huangzhong Agricultural University;Xinjiang Tiankang Animal Science Bio-technology CO.,LTD;Hongqi farm production department,Third Divisions,Xinjiang Production and Construction Corps;;Prevalent Trends and Countermeasures of Peste Des Petits Ruminants in China[J];动物医学进展;2015-01
9 Wang Miao;Wang Jingfei;Zhang Junfeng;Sun Gang;Heilongjiang Animal Disease Prevention and Control Center;Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences;;Investigation of an Introduced Peste des Petits Ruminants Outbreak in N County,Heilongjiang Province[J];中国动物检疫;2014-11
10 HE Jiong-jie;HAN Qing-yan;TANG Hao;LI Yong-sheng;Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS;Gansu Animal Disease Prevention and Control Center Gansu Province;;The Analysis of Occurrence Trend of Peste des Petits Ruminants in Gansu Province[J];畜牧兽医杂志;2014-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LU Yan-mei1,2,ZHANG Jin-wen1,2,WEI Yu-jie3,WEI Gui-min1,2,ZHANG Yan-hong4,GAO Yi-feng1,2(1.Gansu Key Lab of Crop Improvement & Germplasm Enhancement,Lanzhou 730070,China;2.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;3.Agricultural Research Academy of Gansu Reclamation,Lanzhou 730070,China;4.Gansu College of Traditional Chinese Medicine,Lanzhou 730070,China);Construction and tobacco transformation of COR and BBE genes hairpin RNA vector of Papaver somniferum[J];药学学报;2013-07
2 LI Xiaoming1,LIN Wei1,ZHANG Xiang1,WANG Jiang1,ZHANG Jing2,ZHANG Jian2,HAN Tenglong2,YAO Libo2 1Department of Neurosurgery,Xijing Hospital;2Department of Biochemistry and Molecular Biology,School of Basic Medicine,Fourth Military Medical University,Xi'an710032,China;Construction of lentiviral expression vector of Dec1 by Gateway~(TM) system[J];中华神经外科疾病研究杂志;2013-02
3 FENG Guang-xiu,CHEN Hui(Plant Physiology Laboratory of College of Life Science,Shan Xi Normal University,Linfen 041000,China);Construction of RNAi vector of OsNCED3 gene[J];生物学杂志;2012-01
4 XU Hong-li,YI Li,WANG Jian-ke,YANG Shen,CHENG Shi-peng(Institute of Special Wild Economic Animal and Plant Science,Chinese Academy of Agricultural Sciences,Jilin 132109,China);Detection of canine distemper virus in blood and feces of the infected dogs by real-time PCR[J];中国预防兽医学报;2012-01
5 Jun Chen,Xiu Liang and Pei-fu Chen **(College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China);Canine Distemper Virus Utilizes Different Receptors to Infect Chicken Embryo Fibroblasts and Vero cells[J];Virologica Sinica;2011-02
6 REN Chao1,HUO Gui-tao2,CHEN Yi-shan1,CHEN Li1,ZHANG Lan-lan1,LI Jia2,CHEN Wu1,ZHAO De-ming2(1.Beijing Key Laboratory of TCVM,Beijing,102206,China;2.China Agricnlture University Beijing,102206,China);Comparative Study on the Cell Sensitivity of Canine Distemper Virus in Vero and Vero-dst Cell Lines[J];中国比较医学杂志;2011-01
7 ZHAO Jianjun1, ZHANG Hailing1, GAO Han1, ZHAO Chunfei1, CHAI Xiuli1, CHEN Tao1, 2, YAN Xijun1, WU Wei1(1 Division of Zoonosis, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Jilin 132109, China) (2 College of Animal Sciences, Jilin Agricultural University, Changchun 130118,China);Molecular cloning and eukaryotic expression of Canine distemper virus receptor gene SLAM of fox, raccoon dog and mink[J];兽类学报;2010-01
8 WANG Junwei1,REN Weijie1,JIANG Ping2,WANG Zhiliang1,GUO Peiyuan3(1 National Diagnostic Center for Exotic Animal Diseases,China Animal Health and Epidemiology Center,Qingdao 266114,China)(2 College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)(3 College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China);Clinical and pathological observations of mink,fox and raccoon dog canine distemper[J];兽类学报;2009-01
9 SHI Hong-xing,ZHANG Xiao-rong,WU Yan-tao(Key Laboratory of Animal Infectious Disease,Ministry of Agriculture of PRC,Yangzhou 225009,China);The Construction of Vero Cell Line Expressing Canine CD150 Gene[J];中国比较医学杂志;2008-11
10 Jianjun Zhao1,Xijun Yan1,Wei Wu1,2 (1Division of Zoonosis,Institute of Special Economic Animal and Plant Sciences,Chinese Academy of Agricultural Sciences,Jilin 132109,China) (2Jilin Zhongte Biotechnological Co. Ltd,Jilin 132109,China);Genetic variations and cellular receptors of Canine distemper virus——A review[J];微生物学报;2008-07
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved