Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Animal Husbandry & Veterinary Medicine》 2003-01
Add to Favorite Get Latest Update

Indirect ELISA for detection of antibody to PRV inactirated vaccine

CUI Bao an,YANG Ming fan,LI Rui fang,ZHANG Su mei,WANG Xue bin,WANG Li (College of Animal Husbandry and Veterinary Medicine,Henan Agric Univ,Zhengzhou 450002,China)  
An indirect ELISA was established for detection of antibody against PRV in goat sera, employing the antigen which was the sediment through different centrifugal speed and was used to monitor the antibody dynamic regularity of goats after being immunited with inactivated vaccine. The well of an ELISA plate were coated with 0 4 μg antigen. The working titer of conjugate was 1∶800 dilution and the working time is 60 min. 4% was chosen for blocking agent. The time for antigen and antibody inter working was chosen to be 60 min. The result showed that indirect ELISA appeared correspondence in its specificity, sensitivity and repeatability test, and relatively practical for antibody surveillance of PRV.
【CateGory Index】: S852
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 10 Hits
1 TIAN Shu-fei et al(College of Animal Science,Hebei North University,Yangyuan,Zhangjiakou,Hebei 075000);Research Advances and the Control of Riemerella anatipestifer Pathogen[J];Journal of Anhui Agricultural Sciences;2007-20
2 LU Dian hong,ZHANG Yu jin,WANG Gang (Veterinary Medicine Institute,Guangdong Academy of Agicultural Sciences,Guangzhou 510640,China);Advances in Study of Riemerella Anatipestifersis[J];Progress In Veterinary Medicine;2000-S1
3 DENG Zhi-bang ( College of Biological Resources and Environmental Science, Jishou University, Zhangjiajie,Hunan, 427000,China);A Survey of Infectious Serositis of Duck[J];Progress In Veterinary Medicine;2004-06
4 CAI Xiu-lei~1,2,WEI Qiang~2,BAO Guo-lian~2,HUO Cui-mei~1,2,JI Quan-an~2,ZHANG Xian-fu~2(1.College of Animal Science and Technology,Shandong Agricultural University,Tai'an,Shandong,271018,China;2.The Research Institut of Animal Husbandry and Veterinary Medicine,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang,320021,China);Progress on the Lab-diagnosis of Riemerella anatipestifer[J];Progress in Veterinary Medicine;2006-05
5 LIU Chuan-hua1,ZHAO Yan-li2,WANG Yu-hai1,MA Shui-li1(1.Shandong Mengyin County Animal Husbandry Bureau,Mengyin,Shandong,276200,China;2.Shandong Liangshan County Animal Husbandry Bureau,Liangshan,Shandong,272600,China);Development of the Research on Riemerella anatipestifer[J];Progress in Veterinary Medicine;2008-05
6 CHENG Long-fei~(1,2), SHI Shao-hua~1, FU Guang-hua~1, LI Wen-yang~1, PENG Chun-xiang~1, HUANG Yu~1, CHEN Pu-yan~2(1. Animal Husbandary and Veterinary Institue, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China; 2. Key Laboratory of Animal Disease Diagnosis and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China);Detection of the antibody against Riemerella anatipestifer Serotype 2 by indirect enzyme-linked immunosorbent assay[J];Fujian Journal of Agricultural Sciences;2006-02
7 LI Rui fang,CUI Bao an (Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China);Recent advance in the research on bovine pseudorabies and the prospect analysis of immunology advance[J];JOURNAL OF HENAN AGRICULTURAL UNIVERSITY;1999-04
8 LI Rui fang, ZHANG Su mei, YANG Ming fan, CUI Bao an (Engineering College of Animal Husbandry and Veterinary Science, Henan Agicultural University,Zhengzhou 450002,China);Studies on dynamic regularity of antibody after immunization of cows and goats with different assistant PRV inactive vaccines[J];Journal of Henan Agricultural University;2001-03
10 HUANG Qing-zhou,GUO Ding-zhang,DAN Qi-ling,LING Ming-ming,WU Li (Sichuan Institute of Animal Husbandry and Veterinary Medicine,Chongqing Rongchang402460,China);the RA was Separated and Identified,Affected on Medicine in Sichuan and Chongqing[J];Sichuan Animal and Veterin Sciences;2002-01
Chinese Journal Full-text Database 10 Hits
1 Chen Boquan Wu Meiying Ye Qunrui (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);COMPARATIVE STUDIES OF DIFFERENT PURIFICATION METHODS FOR MONOCLONAL ANTIBODY ( McAb )[J];Chinese Journal of Virology;1990-02
2 WANG Ai-hua~(1,2),CHEN Long-bin~1,SU Xiao-geng~1,SUN Ji-guo~1(1.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071001,China;2.Department of Animal Medicine,Hebei North University,Zhangjiakou 075000,China);Study on double sandwich ELISA for the detection of egg drop syndrome-76 virus in chicken[J];Journal of Agricultural University of Hebei;2005-05
3 LIU Jian-jie,WU Bin,CHENG Huan-chun (College of Animal science and vete rinar y Medicine Huazhong Agricultural Univers ity,Wuhan,430070,China);Progress of Research on the Toxigenic Pasteurella Multocida of Porcine Atrophic Rhinitis[J];Progress In Veterinary Medicine;2002-01
4 WANG Quan-xi ~1,WU Yi-jian ~1,CHEN Zhi-hua ~2,LI Guo-ping~1,WU Bao-cheng~1 (1.College of Animal Science in FAFU, Fuzhou, Fujian,350002,China; 2.Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, Fujian,350001,China);Study on the Establishment of Indirecting ELISA for Detecing the Muscovy Duck Antibody of Reovirus Infection[J];Progress In Veterinary Medicine;2005-08
5 ZHANG Xin-jun,SHEN Xiao-bing (1. Huazhong Agricultural University , Wuhan 430070; 2.Southeast University,Nanjing 210000);Influence of RB . KSN Propolis Tablet on Antibody Synthesis of B Cell in Kunming Mice[J];Journal of Bee;2005-10
6 SUN Jun-ling~1,XUE Jia-bin~2,HU Yuan-liang~(1*),WANG De-yun~1,ZHANG Bao-kang~1,LIU Jia-guo~1(1.Institute of Traditional Chinese Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Veterinary Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Adjuvanticity of compound Chinese herbal medicinal ingredients and their efficacy comparison with compound Chinese herbal medicines[J];Journal of Nanjing Agricultural University;2005-04
7 Yang Jianmin Qin Zhuoming Zhao Jixun Su Jingliang (College of Veterinary Medicine,China Agricultural University, Beijing100094, China);Establishment of Direct Dot-ELISA Based on McAb for Detection of AEV Antigen[J];Journal of China Agricultural University;2002-03
8 YUE Hua,LUO Wei,TANG Cheng,YU Xue-hui ,DENG Yi-ke,LIU Qun(The Collage of Animal Science and Technique in South-West University for Nationality,Sichuan Chengdu610041,China);The Study of "Infectious Swollen Head"in Duck[J];Sichuan Animal and Veterin Sciences;2002-08
9 QI Xue-feng,YANG Xiao-yan,LUO Wei,YUE Hua,LIU Nei-sheng(Department of Life Science and Technology,Southwest University for Nationalities,Sichuan Chengdu610041,China);Differentiating Dignose of Duck Plague and“Infectious Swollen Head”by PCR[J];Sichuan Animal and Veterinary Sciences;2003-10
10 LIU Yuan-fa,WANG Xing-guo,JIN Qing-zhe(College of Food Science and Technology,Southern Yangtze University,Wuxi 214036,China);Extraction of Flavone Analogues from Propolis Using Ultrasound[J];Food Science;2004-06
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 XIE Zhi-xun1,QIN Yü2,XIE Li-ji1,LIU Jia-bo1,DENG Xian-wen1,PANG Yao-shan1,XIE Zhi-qin1,TANG Xiao-fei1(1.Guangxi Veterinary Research Institute,Nanning 530001,China;2.College of Animal Science and Technology,Guangxi University,Nanning 530005,China);Development of an indirect ELISA based on antigen of recombinant σ_3 protein of avian reovirus[J];Animal Husbandry & Veterinary Medicine;2007-11
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved