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《Animal Husbandry & Veterinary Medicine》 2003-01
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Indirect ELISA for detection of antibody to PRV inactirated vaccine

CUI Bao an,YANG Ming fan,LI Rui fang,ZHANG Su mei,WANG Xue bin,WANG Li (College of Animal Husbandry and Veterinary Medicine,Henan Agric Univ,Zhengzhou 450002,China)  
An indirect ELISA was established for detection of antibody against PRV in goat sera, employing the antigen which was the sediment through different centrifugal speed and was used to monitor the antibody dynamic regularity of goats after being immunited with inactivated vaccine. The well of an ELISA plate were coated with 0 4 μg antigen. The working titer of conjugate was 1∶800 dilution and the working time is 60 min. 4% was chosen for blocking agent. The time for antigen and antibody inter working was chosen to be 60 min. The result showed that indirect ELISA appeared correspondence in its specificity, sensitivity and repeatability test, and relatively practical for antibody surveillance of PRV.
【CateGory Index】: S852
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 TIAN Shu-fei et al(College of Animal Science,Hebei North University,Yangyuan,Zhangjiakou,Hebei 075000);Research Advances and the Control of Riemerella anatipestifer Pathogen[J];Journal of Anhui Agricultural Sciences;2007-20
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1 XIE Zhi-xun1,QIN Yü2,XIE Li-ji1,LIU Jia-bo1,DENG Xian-wen1,PANG Yao-shan1,XIE Zhi-qin1,TANG Xiao-fei1(1.Guangxi Veterinary Research Institute,Nanning 530001,China;2.College of Animal Science and Technology,Guangxi University,Nanning 530005,China);Development of an indirect ELISA based on antigen of recombinant σ_3 protein of avian reovirus[J];Animal Husbandry & Veterinary Medicine;2007-11
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