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《Animal Husbandry & Veterinary Medicine》 2019-01
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The effects of histone deacetylation regulation by HDAC1 and HDAC2 in mouse oocytes

SUN Xiaofan;LEI Chuqiao;LIU Honglin;ZHANG Xue;College of Animal Science and Technology,Nanjing Agricultural University;  
The aim of this research was to explore the regulatory effects of HDAC1 ( histone deacetylase 1,HDAC1) and HDAC2 ( histone deacetylase 2) on histone deacetylation during the oocyte meiophase. Firstly,the immunofluorescence technique was used in the experiment to detect the expression distribution of HDAC1 and HDAC2 in somatic cells and oocytes of different phases. Then,different inhibitors were added to suppress HDAC1 and HDAC2 to further observe their effects on histone deacetylation. The results showed that,during interphase,both HDAC1 and HDAC2 were distributed in the nucleus of somatic cells and oocytes,but only HDAC1 was co-located with chromosome on oocytes during division stage; and when the enzyme activity was inhibited,the co-locat ion disappeared. Adding TSA ( Trichostatin A,HDACs broad-spectrum inhibitor),Pyroxamide ( HDAC1 specific inhibitor),and Santacruzamate A ( HDAC2 specific inhibitor) to mouse in vitro maturation medium,we found that both Pyroxamide and Santacruzamate A significantly inhibited the deacetylation of histone in the process of meiosis in oocytes,but the inhibitory effect was not broad-spectrum. The present study showed that both HDAC1 and HDAC2 had histone deacetylation effects in the meiotic histone deacetylation in mouse oocytes,but they do not play all the roles,and that,compared with HDAC2,HDAC1 played a major role in this process. These results provided a theoretical reference for exploration into histone deacetylation mechanism.
【Fund】: 国家大学生研究训练计划(201710307035)
【CateGory Index】: S814
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