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《Animal Husbandry & Veterinary Medicine》 2019-01
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The preparation and identification of polyclonal antibody against FMDV 3C protein

CHEN Fei;WU Xiangju;QI Jing;CHENG Ziqiang;DU Yijun;College of Veterinary Medicine,Shandong Agricultural University;Shandong Province Key Laboratory of Animal Disease Control and Breeding;Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences;  
In this study,the FMDV 3C gene was amplified and cloned into a p ET-28 a vector to construct the prokaryotic expression plasmid p ET-28a-3C,which was further transformed into the Escherichia coli BL21 to induce expression. We used Ni-NTA agarose affinity chromatography and Millipore ultrafiltration tube to purify the 3C fusion protein. Then,New Zealand white rabbits were immunized with the purified recombinant protein to produce polyclonal antibodies. ELISA was used to detect the titer and Western blotting was used to analyze the specificity of the polyclonal antibody. The polyclonal antibody was further applied to detect the eukaryotic expression of the 3C protein by IFA and Western blotting. The results of ELISA showed that the titer of the 3C protein polyclonal antibody was as high as 1: 256 000. The polyclonal antibody detected the localization of the 3C protein in cells by IFA. The over-expressed 3C protein in cells could also be detected by Western blotting using the polyclonal antibody. The successful preparation of FMDV 3C polyclonal antibody here provided an important test tool for relevant study of FMDV and its 3C protein.
【Fund】: 国家重点研发计划课题(2016YFD0501505);; 泰山学者青年专家(tsqn20161057);; 山东省农业科学院农业科技创新工程(CXGC2016C07)
【CateGory Index】: S852.65
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 YANG Bin1,2,LIU Ji-xing2(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gausu,730070,China;2.Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,CAAS,Lanzhou,Gansu,730046,China);Review of the Structure and Function of the Foot-and-mouth disease virus 3C Protease[J];动物医学进展;2007-09
2 TIAN Chun-jian,BI Ying-zuo,CAO Yong-chang,XIE Qing-mei (College of Animal Science, South China Agricultural University,Guangzhou 510642,China);T4 bacteriophage surface display of foot-and-mouth disease virus 3 C protease[J];中国兽医科学;2006-07
3 LU Zeng-jun, CAO Yi-mei, LIU Zai-xin, XIE Qing-ge (Key Laboratory of Animal Virology, Ministry of Agriculture/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China);Construction of recombinant adenoviral pShuttle-CMV plasmid containing multiple genes of FMDV[J];中国兽医科技;2004-02
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 LIU Yongjuan;ZHANG Fengfeng;TU Huilin;HE Xiaohua;LIU Wanhong;Pathogens and Infectious Diseases Institute, School of Basic Medical Sciences, Wuhan University;Hubei Province Key Laboratory of Allergy and Immunology;;Research progress of 2A protein of enterovirus 71[J];生命的化学;2014-06
2 TIAN Chun-jian,BI Ying-zuo,CAO Yong-chang,XIE Qing-mei (College of Animal Science, South China Agricultural University,Guangzhou 510642,China);T4 bacteriophage surface display of foot-and-mouth disease virus 3 C protease[J];中国兽医科学;2006-07
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 WU Jian-min~(1,4),REN Zhao-jun~(2),YU Xing-long~(1),ZHANG Nian-zu~(3),TU Chang-chun~(1*) (1.The Military Veterinary Institute,Academy of Military Medicine of PLA,Changchun 130062,China;2.Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine,Baltimore,MD 21201- 1503,USA;3.Yunnan Tropical and Subtropical Animal Viral Diseases Laboratory,Kunming 650224, China;4.Guangxi Veterinary Institute,Nanning 530001,China);CSFV E2 Gene Display on Bacteriophage T4 and the Immunological Activity of Its Expression Product[J];中国兽医学报;2004-06
2 LIU You, BI Ying-zuo, CAO Yong-chang, MA Jing-yun, XIAO Jun-fang (College of Animal Science, South China Agricultural University, Guangzhou 510640,China);Display of globular domain of HN protein of Newcastle disease virus on bacteriophage T4 capsid surface[J];中国兽医科技;2004-08
3 LU Zeng-jun, CAO Yi-mei, LIU Zai-xin, XIE Qing-ge (Key Laboratory of Animal Virology, Ministry of Agriculture/Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China);Construction of recombinant adenoviral pShuttle-CMV plasmid containing multiple genes of FMDV[J];中国兽医科技;2004-02
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