AN ANALYSIS ON AFFECTED FACTORS DURING FREEZING THE SPERM OF GOAT
Wang Chao; Zhou Qingyu; Ding Jiatong(Lianyungang Egg and Livestock Development Corporation;Agricultural College, Yangzhou University)
In this test, three kinds of diluents, three kinds of glycerine concentration, three kinds of yolk concentration and three kinds of starting frozen temperature were used in freezing and defrosting the sperm of goat at designed proccdure,checking gradually the activity of sperm and living sperm percentage. We observed the spcrm by scrccning supcrmicrostructutc with EM after collection of spcrm. before freezing and after defrosting. All data were analysed by single factor analysis of significant difference. It showed that among three dilnents, diluent C is worst,diluent A and B were significant difference (P0.05),dilucnt A is better,It also showed that the living activity of frozen semena in three kinds of glycerine coneentration were significant diffcrcncc (P0.05), 6% is best. The rate of spcrm acrosoine damage was developing with increase of glycctinc conccntration. The diffcrcncc uf different yolk concentration and starting frozen temperature weren't significant (P0.05). Two superovulation ewe were in seminated with the frozcn semena involed 6% glycerine. It showcd that fertilization ratc is 97%, embryo growth ratc is 87. 3% aftcr flushing oosperm and observing it by EM at the 8th day after oestrus.
【CateGory Index】： S827.34