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《Medical Journal of National Defending Forces in Southwest China》 2014-01
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Liquid phase chip inspection of the effects of humidity environment on the expression of T lymphocyte in rats

Song Fuqiang;Guo Wenqiong;Li Kun;Yang Yun;Guo Xin;Li Wei;Hu Yonghe;Central Lab,General Hospital of Chengdu Military Command;School of Nursing,Chengdu Medical College;  
Objective To detect the expression of T lymphocyte in rats' blood serum by liquid phase chip technology,and to discuss the influence of high humidity environment on rats' body functions. Methods Thirty two SD rats were randomly divided into high humidity and normal groups with 16 ones in each group. Climatic cabinet was used to simulate the high humidity environment. The temperature was 25 ℃,and the relative humidity was 90%-95% RH. The rats of the high humidity group were placed in the cabinet for 10 h everyday. In the rest of the day,they were feed in the same clean environment as the normal group with common temperature and humidity( temperature of 25 ℃ and relative humidity of 45%-50% RH) continuously for 20 days. After the establishment of the animal model,the blood samples were obtained from the abdominal aorta of the rats in order to isolate the serum. Luminex200 system was used to detect the expression of nine cytokines. Results Compared with the indexes of the normal group,the levels of serum IL-1α,IL-1β, IL-2,IL-6,and IL-10 in the high humidity group significantly increased( P 0. 05 or P 0. 01); FNγ obviously decreased( P 0. 01); the TNFα,EGF,and VEGF did not change significantly( P 0. 05). Conclusion High humidity can change the expression of serum cytokines in rats. The Th1 / Th2 cytokine balance is broken,which results in the changes of the rats' immune homeostasis status.
【Fund】: 全军医学科技“十二五”重点项目(BWS11J067)
【CateGory Index】: R285.5
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