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《Medical Journal of National Defending Forces in Southwest China》 2014-01
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Suppression of gastric cancer cell growth by tumor-targeted adenovirus mediated IL-24 gene

Cao Yaling;Huang Maotao;Huang Yi;Ji Daijin;Feng Zaoming;Li Xuecheng;Chen Ling;Department of Digestion,Hospital 452 of PLA;Department of Digestion,Hospital 324 of PLA;Emei Sanatorium of Chengdu Military Command;  
Objective To discuss the effects of human telomerase reverse transcriptase( hTERT) promoter targeted recombinant adenoviral vector carrying human IL-24 gene( Ad-phTERT-IL-24) on the proliferation and infestation ability of SGC-7901 human gastric cancer cells. Methods SGC-7901 tumor cells were infected by Ad-phTERT-IL-24. IL-24 expression level was examined by RT-qPCR and Western blot. CCK8 assay was used to detect the cell growth curve. Transwell camber infestation experiment was carried out to evaluate the invasion capability of SGC-7901 tumor cells. Results The expression of IL-24 in the SGC-7901 cells infected with Ad-phTERT-IL-24 significantly increased compared with the cells treated with PBS instead of virus liquid( P 0. 01),the absorbance decreased significantly since the fifth and sixth day( P 0. 05). Meanwhile the invasion ability also decreased significantly( P 0. 05). Conclusion Ad-phTERT-IL-24 can effectively up-regulate the IL-24 expression and furthermore suppress the proliferation and invasion ability of human SGC-7901 gastric cancer cells.
【Fund】: 国家自然科学基金资助课题(30900679);; 重庆市集成示范计划项目(cstc2013jcsf10014);; 重庆市医学科研计划项目(2011-2-587);; 四川省卫生厅科研课题(110484)
【CateGory Index】: R735.2
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