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《Medical Journal of National Defending Forces in Southwest China》 2016-01
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Effects of Schwann cell-derived neurotrophic factor and hepatocyte growth factor on activity of rats' muscle satellite cells in vitro

Wu Jiang;Xu Yongqing;Zhu Min;Zhu Chongtao;Chen Xusheng;Trauma and Orthopaedics Research Institute of PLA, Kunming General Hospital of Chengdu Military Command;  
Objective To study the effects of Schwann cell-derived neurotrophic factor(SDNF) and hepatocyte growth factor on the proliferation and differentiation of rats' muscle satellite cells(MSCs) in vitro. Methods Rats' muscle satellite cell culturing systems in vitro were established and divided into four groups according to the different culture media: group A: 10% FBS; group B:10%FBS+400 ng/ml SDNF; group C: 10% FBS+200 ng/ml HGF; group D: 10%FBS+400 ng/ml SDNF+200 ng/ml HGF. MSCs were treated with various SDNF concentrations culture medium. The shape of MSCs was observed dynamically and cell growth curve, and the formation rate of myotube was determined. Results The culturing media containing different factors all could promote the proliferation of MSCs. The MTT detection results indicated that the cell growth in group C and D was prosperous, but there was no significant difference in the growth among group B, C, and D(P 0.05). However, the cellular proliferation in group A was slower than that in the other three groups(P 0.05). The other three groups' cell fusion numbers increased significantly compared with that in group A, and the formation of myotube in group B and D was fairly obvious(P 0.01). Conclusion HGF plays major role in MSCs activation and proliferation, while SDNF can regulate the differentiation of rats' MSCs in vitro. The effects of the two kinds of factors interact and promote each other.
【Fund】: 云南省应用科学基金资助项目(2010ZC180)
【CateGory Index】: R329.2
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