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《Medical Journal of National Defending Forces in Southwest China》 2016-01
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Cytotoxicity assay of polyvinyl alcohol/platelet-rich fibrin wound dressing in vitro

Xu Fangfang;Dai Taiqiang;Xu Haiyan;An Ran;Liu Bin;State Key Laboratory of Military Stomatology, Department of Dentofacial Surgery, Dental Hospital of the Fourth Military Medical University;State Key Laboratory of Military Stomatology, Laboratory Animal Center, Dental Hospital of the Fourth Military Medical University;  
Objective To detect the cytotoxicity of polyvinyl alcohol/platelet-rich fibrin(PVA/PRF) wound dressing. Methods PVA/PRF wound dressing was prepared and co-cultured through L929 cells and dressing leaching liquor of different concentration.The relative growth rate was detected by MTT assay. Scanning electron microscope and fluorescence microscope were used to observe the cellular shapes. Results Cell growth forms were in good condition in the groups of different concentration. L929 cells tightly adhered to the material with normal shape. The toxicity gradation of all the experimental groups was 0-1. Conclusion PVA/PRF has no cytotoxicity.
【Fund】: 国家自然科学基金资助课题(31170942)
【CateGory Index】: R318.08
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