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《Medical Journal of National Defending Forces in Southwest China》 2016-01
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Simultaneous determination of two components content in compound Kangfu liniment by HPLC method

He Deyun;Zhong Yuangao;Liao Haiyan;Shen Dongni;Han Huanhuan;Zhang Tao;Li Jia;Institute for Drug and Instrument Control of Chengdu Military Command;  
Objective To establish a determination method of salicylic acid and dexamethasone acetate content in compound Kangfu liniment by HPLC. Methods Agilent 1100 HPLC was used to simultaneously determinate the content of salicylic acid and dexamethasone acetate in compound Kangfu liniment. The column was agilent 5 HC-C18(2)(4.6 mm×250 mm; 5 μm); mobile phase was 0.025 mol/L potassium dihydrogen phosphate(p H =3.0) ∶acetonitrile(60 ∶40); the detection wavelength was 240 nm; the column temperature was 30 ℃; and the injection volume was 10 μl. Results Salicylic acid had a good linear correlation with the peak area within the range of 1.068 μg to 10.68 μg, and the regression equation was Y =1887X +325.74(r =0.9997). Dexamethasone acetate had a good linear correlation with the peak area within the range of 0.0202 μg to 0.2018 μg, and the regression equation was Y=2055.4X+0.1304(r=1). The average sample recovery rates of salicylic acid and dexamethasone acetate were 100.91%( RSD=0.38%) and 101.67%(RSD =0.94%), respectively. Conclusion HPLC method has the advantages of easy operation and accurate quantitation, which is suitable for the simultaneous content determination of salicylic acid and dexamethasone acetate in compound Kangfu liniment.
【CateGory Index】: R927.2;O657.72
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