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《Journal of Southwest University(Natural Science Edition)》 2019-01
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Characterization and Expression Analysis of Chitinase Family Genes in Sclerotinia sclerotiorum

DU Jiao;WANG Ya-bo;XIAO Ji-fen;LI Xue-hua;YANG Yu-heng;BI Chao-wei;YU Yang;School of Plant Protection,Southwest University;Liangshan National Normal School;  
The carpogenic germination of sclerotia for Sclerotinia sclerotiorumis a key step of crop sclerotiniose infection cycle.Studying the molecular mechanism involved in the carpogenic germination of scleortia will provide clues to control the sclerotiniose safely.In this study,chitinase family proteins of S.sclerotiorum were analyzed with the bioinformation approach,and the expression levels of chitinase genes during the carpogenic germination of sclerotia were determined with RT-PCR method.Bioinformatics analysis showed that 12 genes were predicted to encode chitinase in S.sclerotiorum.All of them had GH18 domains and some also had a chitin-binding domain and a cellulose-binding domain.All the chitinases were hydrophilic except SS1G_11212.Ten chitinases were predicted to be located outside the cell,and the others were located in the nucleus cytoplasm.Phylogenetic tree analysis showed that the 12 chitinases could be divided into two categories,of which 3with yeast chitinase CTS1 were clustered together and the rest with CTS2 were clustered together.The expression level of the 11 chitinase genes changed during the carpogenic germination of sclerotia,except SS1G_00773,suggesting that chitinase family proteins are involved in the carpogenic germination of sclerotia for S.sclerotiorum.
【Fund】: 重庆市基础与前沿研究一般项目(cstc2017jcyjAX0096);; 中央高校基本科研业务费“创新团队”专项(XDJK2017A006)
【CateGory Index】: S432.44
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