Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
Add to Favorite Get Latest Update


Wang Zhongkang; Luo Huaihai *; Shu Zhengyi (Dept. of Plant Protection, Southwest Agr. Univ., Chongqing 400716) Shen Jun; Bai Xianhui (Animal & Plant Quarantine Bureau, Chongqing 400020) Xie Zhongyou; Fan Shengyu (Station of Plant Prot., Chongqing  
The capability of rapid detection of Xanthomonas axonopodis pv. citri with Dot Immunobinding Assay (DIA) was determined on the minipore filter of nitrocellulose. Effective enhancement of antiserum specificity through cross absorption of saprophytic bacteria from citrus leave and xanthomonads with colosely relationship resulted in increasing specificity of polyclonal antibodies. Lowest limitation of detection for target pathogen was 1×10 4 CFU ml -1 , Positive reactions obtained from 128 fold dilutions of soaking solution of disease citrus leave. The filter paper background can be improved via adding 0.1% Triton X-100 in TBS buffer. All 26 isolates of X.a.pv.citri from Southern China showed positive reaction with DIA but the negative controls. Detection of X.a.pv.citri with DIA indicated that there has 31.82% average positive based on 518 samples of citrus asymptomatic materials from 38 counties, 8 provinces of Southern China.
【CateGory Index】: S436.661.19
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 WANG Zhong-kang1,XIA Yu-xian1,SUN Xian-yun2,ZHOU Chang-yong2,YIN You-ping1( Research Center of Genetic Engineering,Chongqing University,Chongqing 400030;1 2College of Plant Protection,Southwest Agricultural University,Chongqing 400716 );Comparison of PCR,DIA and Pathogenicity Assay for Detection of Xanthomonas axonopodis pv. citri,the Causal Agent of Citrus Bacterial Canker Disease[J];Scientia Agricultura Sinica;2004-11
2 Xiong Shu,Wang Zhongkang,Lu Xiaolin,Yin Youping(College of Life Sciences,Chongqing University,Chongqing 400030,China);Establishment of EMA-PCR detection method of viable cells of Xanthomonas citri subsp. citri[J];Plant Protection;2013-04
China Proceedings of conference Full-text Database 1 Hits
1 ;Detection of citrus bacterial cankerdisease by fluorescence quantitative PCR[A];[C];2006
Chinese Journal Full-text Database 10 Hits
1 CHEN Jian-shan et al (Environment and Plant Protection College,SCUTA,Danzhou,Hainan 571737);Application of ITS Sequences in Fungi Classification and Identification[J];Journal of Anhui Agricultural Sciences;2007-13
2 KAN Chun-yue et al(Shanghai Ocean University,Shanghai 201300);Progress of Detecting Plant Seed-borne Diseases[J];Journal of Anhui Agricultural Sciences;2010-15
3 ZHANG Xiao-lei1,TAN Gen-jia1,WEI Mei-sheng2,ZHANG Yong-jiang2,LI Gui-fen2(1.School of Plant Protection,Anhui Agricultural University,Hefei 230036;2.Institute of Animal and Plant Quarantine,Chinese Academy of Inspection and Quarantine,Beijing 100029);Detection of southern bean mosaic virus and tobacco ringspot virus in soybean seed by GICA-RT-PCR[J];Journal of Anhui Agricultural University;2009-02
4 Chen Jing;Hu Weizhen;Yu Jialing;Han Chenggui A rapid method was developed for the detection of Tomato Ringspot Virus(TomRSV)based on the reverse transcription and polymeras chain reaction(RT-PCR).The amplified frag- ment of about 1kb was easily see;RAPID DETECTION OF TOMATO RINGSPOT VIRUS BY THE REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION[J];Chinese Journal of Virology;1996-02
5 LI Qi-ming~1 , MA Xue-jun~(1,2), HOU Yun-de~(1,2)(1.State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100052, China; 2.Beijing Jindike Biotech Institute, Beijing 100176, China);Detection of RNA Virus Gene by Rolling Circle Amplification Method[J];Chinese Journal of Virology;2005-04
6 Li Chenxu;Yin Xiaoguang;Sun Changjie(The School of Preventive Medicine;Hospital of China First Automotile Group Corporation);Research of rapid diagnosis of HCG by immunochromatography[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1998-02
7 ZHAO Chun-yan, YI Shi-hong, LI Fan (Department of Pathogenobiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China);Rapid detection of E. coli O157 : H7 and other Shiga toxin-producing E. coli using ramification amplification method[J];Journal of Jilin University(Medicine Edition);2006-01
8 Zhao Rongle Zheng Guangyu(College of Life Sciences, Beijing Normal University, 100875, Beijing, China);DETECTION OF THREE PLANT VIRUSES USING DOT-IMMUNOBINDING ASSAY[J];Journal of Beijing Normal University(Natural Science);2004-04
9 WU Zhi ming 1, ZHU Shui fang 2, ZHANG Cheng liang 2, TIAN Wen hui 3 (1 Plant Genetic Transformation Center, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhang 050051, China; 2 Plant Quarantine Institute, Ministry of Agric;A rapid method for detection of potato leafroll virus by reverse transcription polymerase chain reaction amplification[J];Journal of Agricultural University of Hebei;2000-04
10 Zhang Qing-jie Zhang Jing-ning Huang Zi-ran(South China Agricultural University,Guangzhou 510642,China) Tan Shi-ci Guo Zhou-yi(South China Normal University,Guangzhou 510630,China);THE BACTERICIDAL EFFECT OF ANTIBACTERIAL PEPTIDE FROM CHINESE OAK SILKWORM TOTHE PATHOGEN OF BACTERIAL ULCER AND YELLOW SHOOT DISEASE IN CITRUS[J];ACTA SERICOLOGICA SINICA;1995-02
China Proceedings of conference Full-text Database 3 Hits
1 ;转抗菌肽D基因沙田柚对柑橘溃疡病抗性的评价[A];[C];2006
2 ZHANG Liping, WANG Zhongkang~*, YIN Youping, XIA Yuxian (The Center for Genetic Engineering Research, Key Lab for Biomechanics & Tissue Engineering under the State Ministry of Education, Chongqing University, Chongqing 400044);Comparison of Several DNA Extraction Methods of Huanglongbing Disease of Citrus (Liberobacter asiaticus) Suitable for PCR Assay[A];[C];2004
3 ;Detection of citrus bacterial cankerdisease by fluorescence quantitative PCR[A];[C];2006
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 ZHANG Hua-peng,ZHANG Jian-feng,LIU Jun-ying,WANG Cong-cong(College of Agricultural and Plant Protection,Qingdao Agricultural University, Plant Virus Laboratory,Qingdao 266109,China);Triplex-RT-PCR Detection of PVY,PVS and PLRV in Potato[J];Acta Agriculturae Boreali-Sinica;2011-05
2 Yin Youping Li Meng Yu Hong Wu Yujia Wang Zhongkang Bioengineering College of Chongqing University,Key Laboratory of Gene Function and Regulation of Chongqing,Chongqing 400030,China;Reconstruction,Expression and Characterization of Disulfide-stabilized Single Chain Variable Fragment to Xanthomonas axonopodis pv. citri[J];Journal of Agricultural Biotechnology;2011-01
3 NIAN Si-ji, YIN You-ping, YUAN Qing, XIA Yu-xian, WANG Zhong-kang LI Min-hui(College of Bioengineering of Chongqing University, Genetic Engineering Research Centre of Chongqing University,Key Laboratory of Gene Function and Regulation at Chongqing, Chongqing University, Chongqing 400030, China);Selection of differential DNA fragment of Tilletia controversa Kühn and establishment of molecular detection approach[J];Acta Microbiologica Sinica;2007-04
4 YAN Jia-wen YANG Li YUAN Fei-rong ZHANG Jia-yin LI Na WANG Xiao-jun DENG Zi-niu;Expression of PthA-nls Gene in Sweet Orange Analyzed by Quantitative Polymerase Chain Reaction[J];Chinese Horticulture Abstracts;2010-04
5 ZHANG Quan SUN Huai-chang (1.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China) ZHU Hong-fei (2.Institute of Animal Science and Veterinary Medicine,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Development of conventional PCR and Real-time PCR for detection of african swine fever virus[J];Chinese Journal of Preventive Veterinary Medicine;2007-06
6 Yin Youping,Wu Yujia,Yu Hong,Li Meng,Wang Zhongkang (Bioengineering College,Chongqing University,Chongqing 400030,China);A gold immunochromatographic assay for the detection of Xanthomonas citri pv. citri[J];Plant Protection;2012-02
China Proceedings of conference Full-text Database 1 Hits
1 ;Detection of citrus bacterial cankerdisease by fluorescence quantitative PCR[A];[C];2006
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved