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《Journal of Xianning College(Medical Sciences)》 2010-01
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Effect of Dual Silencing of IGF-1 and EGF Receptors on PCNA Labeling Index and Mitotic Count of Human Nasopharyngeal Carcinoma Xenografts in Nude Mice and Cell Cycle of Nasopharyngeal Carcinoma Cells

LU Shuo,MENG Xiao-ping,ZHOU Xu-hong,et al(Department of Anatomy,Wuhan University Basic Medical School,Wuhan Hubei 430071,China)  
Objective To study the cellular proliferation status of human nasopharyngeal carcinoma xenografts in nude mice and cell cycle of nasopharyngeal carcinoma cells by dual silencing of IGF-1 and EGF receptors(DSIER).Methods All the cases were stained with PCNA on paraffin embedded sections by immunohistochemistry.Mitotic count was done on HE sections corresponding to each cases.Cell cycles of nasopharyngeal cells by DSIER were measured by flow cytometry.The Replication Index(RI) was counted accorrding to cell cycle.Results The mean of PCNA labeling index(PCNA-LI) was 27.19±10.20 in DSIER group,while both of control groups were 36.47±8.63 and 37.07±8.63,respectively.The mean of mitotic count per 10 high power fieldsis was 18.80±7.47 in DSIER group,while both of control groups were 37.53±5.25and 38.03±6.15,repectively.The correlation coefficient between them was 0.505(P0.01) in DSIER group.There is a significantly positive correlation between PCNA-LI and mitotic counts in DSIER group.The RI in DSIER group was 36.2±0.65,while RI of both of control groups was 52.09±0.08 and 55.35±0.08,respectively.The cell rates in G0/G1 phase,S phase and G2/M phase of DSIER group were 63.80±0.64,33.29±0.21 and 2.91±0.04,respectively,while the cell rates in G0/G1 phase,S phase and G2/M phase of both of control groups were 47.91±0.65 and 9.38±0.14,respectively.Conclusion Dual silencing of IGF-1 and EGF receptors has obvious effect on the PCNA labeling index and mitotic count of human nasopharyngeal carcinoma xenografts in nude mice and on cell cycle of nasopharyngeal carcinoma cells.PCNA labeling index,mitotic count and cell cycle can be used to evaluate the cellular proliferation status of Nasopharyngeal carcinoma.
【CateGory Index】: R739.63
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