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《Journal of Xianning University(Medical Sciences)》 2012-01
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Isolation and Identification of Side Population Cells from Human Bladder Transitional Cell Cancer

NING Zhi-feng,JIANG Chun(Department of Human Anatomy,Basic Medical School,Xianning University,Xianning Hubei 437100,China)  
Objective To isolate and identify side population cells from human bladder transitional cell cancer.Methods Dual wavelength FACS was used to sort four fresh bladder transitional cell cancer tissues and three bladder cancer cell lines after Hoechst 33342 labeling and the potential stem cell characteristics of side population cells from T24 were compared according to proliferation,cell cycle distribution,self-renewal,differentiation,sensitivity to chemotherapy drugs and X-ray.Results Several bladder transitional cell cancer cell lines such as T24 and four fresh bladder transitional cell cancer tissues had side population cells of different proportions,but EJ and UM-UC-3 had no side population.Moreover,T24 SP cells had stronger proliferative and colony formation abilities than non-SP cells.SP cells were also more resistant to chemotherapy and radiotherapy,which might be due to expression of the ATP-binding cassette half-transporter,sub-family G,member 2 protein.Cell cycle results suggested that more SP than NSP cells were in the G0/G1 phase(87.4% vs 63.3%,P 0.05) and fewer SP than NSP cells were in S phase(31.1% vs 9.1%,P 0.05).After about 10 days culture in normal condition,SP cells still contained 23.8% of cells that stained poorly with Hoechst 33342.In contrast,NSP cells only contained 2.6% poorly staining cells.RT-PCR results suggested SP cells expressed more ABCG2,Notch-1,β-catenin and SMO than NSP cells.Conclusion Side population cells can be found in human bladder transitional cell cancer and side population cells from human bladder transitional cell cancers cell line T24 harbor stem-like cells.
【CateGory Index】: R737.14
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